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D.C. Wu, L.M. G. Mimica, C.B. Silva, S. Ueda, M.C. Nishiwaki–Dantas, P.E. C. Dantas, V.B. M. Fiorelli, R.Y. Hida; In vitro Antimicrobial Efficacy of Corneal Storage Media Containing Gentamycin and Streptomycin . Invest. Ophthalmol. Vis. Sci. 2006;47(13):2359.
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Evaluate antimicrobial efficacy, in vitro, of corneal storage media with gentamicin and streptomycin (Optisol–GS®, Chiron Intraoptics Inc, Irvine, California) in Staphylococcus aureus (ATCC25923), under simulated environment of corneal preservation.
We investigated contaminated corneal storage media with Staphylococcus aureus stored at 4o C in different moments of incubation (1, 2, 3, 4, 5, 6, 24 e 48 hours; 7 days and 14 days) using a unique bacterial medium system (Sistema Hemobac Trifasico N/A pediátrico®, Probac do Brasil, Sao Paulo, Brasil). This system contains 3 mediums (chocolate agar, Sabouraud agar and carbon dioxide indicator) and a inferior chamber containing a antibiotic inhibitor (activated coal).
After 24 hours, carbon dioxide indicator showed positive results in samples after 1, 2 and 3 hours after contamination and bacterial growth after 1, 2, 3 and 4 hours after contamination. After 48 hours, bacterial growth and carbon dioxide indicator showed positive results in the rest of the samples, except for the sample of 14 days. All samples with bacterial growth were confirmed with bacterioscopy and gram for Staphylococcus aureus.
contaminated corneal preservation media containing gentamycin and streptomycin, in vitro, showed viable Staphylococcus aureus (ATCC25923) within 7 days after preservation.
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