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H. Zhu, F. Stapleton, M.D. P. Willcox; Broad Spectrum of Antibacterial Activity of a New Multi–Purpose Disinfecting Solution . Invest. Ophthalmol. Vis. Sci. 2006;47(13):2413.
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Contact lenses, lens cases, and lens care products are exposed to many possible sources of microbial contamination, including environmental sources, ocular and skin biota. Potential contamination may come from water, air, soil, animals, plants, lens cases, and lens handling. Using a contact lens disinfecting product with a broad spectrum of antimicrobial activity gives more assurance that viable populations of contaminating microbes will be reduced. The aim of the current study was to evaluate the antibacterial efficacy of a new contact lens disinfecting solution against a broad spectrum of bacteria.
A new multi–purpose disinfecting solution (MPDS) was challenged with a broad range of bacterial strains isolated from clinic and environment, including 10 Gram–positive and 8 Gram–negative bacteria. The panel of reference microorganisms recommended by ISO standard were also included. Three different product lots were challenged with at least three separate inocula of each isolate. After exposure to the disinfecting solution for 4h, 6h, or 24h, samples were removed from the product or control solution and plated for survivors using suitable recovery methods. The number of survivors was enumerated.
The new MPDS showed antimicrobial activity against the five reference microorganisms in excess of that recommended by ISO standards. The solution demonstrated a broad spectrum of antibacterial activity, showing a minimum of 3–log reduction in 7 out of 10 Gram–positives and all 8 Gram–negative isolates. The efficacy of the solution was increased with longer disinfection times (p < 0.01).
The new MPDS produced a notable reduction of various clinical and environmental bacteria. The solution may contain antimicrobial agents that can kill an extensive spectrum of bacteria that may contaminate contact lens and solutions.
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