May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
The Pathology of Lens of Perlecan Rescued Mice
I. Masuda; Y. Takada; T. Igarashi; F. Kang; M. Fukushi; T. Hasegawa; Y. Yamada; K.G. Csaky
Author Affiliations & Notes
  • I. Masuda
    National Institutes of Health, National Eye Institute/Section of Retinal Diseases and Therapeutics, Bethesda, MD
    Okayama University Medical School, Dept of Ophthalmology, Okayama, Japan
  • Y. Takada
    NIH, National Institute on Deafness and Other Communication Disorders, Bethesda, MD
  • T. Igarashi
    National Institutes of Health, National Eye Institute/Section of Retinal Diseases and Therapeutics, Bethesda, MD
  • F. Kang
    NIH, National Institute of Dental and Craniofacial Research/Craniofacial Developmental Biology and Regeneration Branch, Bethesda, MD
  • M. Fukushi
    NIH, National Institute of Dental and Craniofacial Research/Craniofacial Developmental Biology and Regeneration Branch, Bethesda, MD
  • T. Hasegawa
    NIH, National Institute of Dental and Craniofacial Research/Craniofacial Developmental Biology and Regeneration Branch, Bethesda, MD
  • Y. Yamada
    NIH, National Institute of Dental and Craniofacial Research/Craniofacial Developmental Biology and Regeneration Branch, Bethesda, MD
  • K.G. Csaky
    National Institutes of Health, National Eye Institute/Section of Retinal Diseases and Therapeutics, Bethesda, MD
  • Footnotes
    Commercial Relationships  I. Masuda, None; Y. Takada, None; T. Igarashi, None; F. Kang, None; M. Fukushi, None; T. Hasegawa, None; Y. Yamada, None; K.G. Csaky, None.
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 2530. doi:
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      I. Masuda, Y. Takada, T. Igarashi, F. Kang, M. Fukushi, T. Hasegawa, Y. Yamada, K.G. Csaky; The Pathology of Lens of Perlecan Rescued Mice . Invest. Ophthalmol. Vis. Sci. 2006;47(13):2530.

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Abstract

Purpose: : Perlecan is a major heparan sulfatrte proteoglycan (HSPG) of basement membranes (BMs) and connective tissue. Heparan sulfate(HS) side chains in the lens capsule has been reported to have a structural function and be essential in the insulation of the lens. The aim of this study is to examine the role for core protein of perlecan in the development of cataract.

Methods: : The paraffin sections were stained by hematoxylin–eosin and PAS method. The paraffin sections of neonatal mouse eyes used for In Situ TUNEL assay. Frozen sections were stained by perlecan primary antibody. The acid DNase in lens was measured. The expression of adhesion molecules(cadherin,integrin) was checked in lens.

Results: : Immunostaining for perlecan confirmed the presence of perlecan in lens in wild mice and perlecan rescued mice. PAS staining cleared the degeneration of lens capsule and the detachment of lens epithelial cells. In situ TUNEL assay and acid DNase assay are on going.

Conclusions: : The core protein of perlecan may play a role in the development of cataract . We will investigate relationship catarct and perlecan still more.

Keywords: none 
© 2006, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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