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S.–M. Lee, H.–S. Park, S.–J. Park, T.–H. Kang, J.–W. Chung, M.–H. Chun, S.–J. Oh; Expression Pattern Of Substance P In The Rat Retina At Normal And At Diabetes . Invest. Ophthalmol. Vis. Sci. 2006;47(13):2576.
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Substance P (sub P) being composed of 11 amino acids sequence is a kind of tachykinin family peptides. It has known that this substance plays a role of neurotransmitter and/or neuromodulator and is a very potent vascular growth factor in the nervous system. Focusing on dual roles of sub P, this study has preliminarily investigated expression pattern of sub P by immunochemistry in the rat retina at normal and at experimental diabetes as a well–studying model of vascular associated ocular diseases.
Diabetic condition was induced by a single injection of streptozotocin in Sprague–Dawley rats aged 8 weeks. The animals showing high blood glucose levels (above 300mg/dl) were cared for 1,4,8 and 12 weeks, respectively. The whole–mounted or sectional preparations of the retinas were used for sub P immunohistochemistry.
Sub P immunoreactivity has been localized in the subsets of amacrine cells in the inner nuclear layer (INL) and displaced amacrine cells in the ganglion cell layer (GCL) in the normal retina. The dendrites from amacrine cells in the INL were ramified with sublaminae 1 and 3, and those from displaced amacrine cells with sublamina 5 of the inner plexiform layer. Sub P immunoreactive neurons in both the INL and the GCL were more densely populated in the superior half and of the retina. During diabetes, the cell number of sub P immunoreactive neurons was recorded from one third to one second of the normal value at 4 weeks diabetes and then increased to three quart to nine tenth of the normal at 12 weeks diabetes.
These results suggest that sub P in the rat retina may function differentially in the superior or the inferior halves, and sub P synthetic pathway in the retinal neurons is irradiated in earlier staged and then restored in later by diabetic injury.
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