Abstract
Purpose: :
To develop a retinal neovascularization experimental model in rabbits using latex angiogenic factor (LAF) intravitreous injection.
Methods: :
LAF was encapsulated in PLGA microspheres. 20 albino New Zealand rabbits were divided in groups of five. Group I received 10µg/0,1ml of LAF intravitreally; group II received 30µg/0,1ml of LAF; group III received 50µg/0,1ml of LAF and group IV received 0,1 ml of microspheres intravitreally, without the angiogenic factor. Fluorescein angiography was performed before the injections and 7, 14, 21and 28 days after the procedures, when the rabbits where euthanized and the eyes processed for light microscopy.
Results: :
All eyes (n=5) from Group I rabbits demonstrated retinal vascular tortuosity, associated with few micro–hemorrhages 14 days after injection. All Group II eyes (n=5) showed vascular changes similar to Group I. Three eyes from group II also developed retinal neovascularization 21 days after injection. All eyes (n=5) from group III rabbits developed important vascular tortuosity and retinal new vessels 2 weeks after injection. We did not observe vascular changes or retinal new vessels in Group IV eyes (n=5). Light microscopy confirmed new vessels visualized on fluorescein angiography.
Conclusions: :
Thirty and fifty micrograms intravitreous injection of latex angiogenic factor induced experimental retinal neovascularization in rabbits.
Keywords: retinal neovascularization • neovascularization • retina