May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Macrophage Activation in the Retina of Vldlr–/– Mice With Subretinal Neovascularization
Author Affiliations & Notes
  • W. Wang
    Department of Ophthalmology, Indiana University, School of Medicine, Indianapolis, IN
  • H. Meng
    Department of Ophthalmology, Indiana University, School of Medicine, Indianapolis, IN
  • W. Hu
    Department of Ophthalmology, Indiana University, School of Medicine, Indianapolis, IN
  • G. Jiang
    Department of Ophthalmology, Indiana University, School of Medicine, Indianapolis, IN
  • X. Qiao
    Department of Ophthalmology, Indiana University, School of Medicine, Indianapolis, IN
  • H. Gao
    Department of Ophthalmology, Indiana University, School of Medicine, Indianapolis, IN
  • Footnotes
    Commercial Relationships  W. Wang, None; H. Meng, None; W. Hu, None; G. Jiang, None; X. Qiao, None; H. Gao, None.
  • Footnotes
    Support  Research to Prevent blindness Foundation and Reeves Foundation
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 2599. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      W. Wang, H. Meng, W. Hu, G. Jiang, X. Qiao, H. Gao; Macrophage Activation in the Retina of Vldlr–/– Mice With Subretinal Neovascularization . Invest. Ophthalmol. Vis. Sci. 2006;47(13):2599.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Subretinal neovascularization (SRN) is a significant feature of VLDL receptor knockout mice (vldlr–/–). We have previously studied the development of SRN, localized VLDLR expression in mouse retina, and examined the mRNA levels of angiogenic factors in vldlr–/– mice. To further characterize the nature of the pathological angiogenic process, various markers were used to determine which cells were activated during the development of SRN in the vldlr–/– mouse.

Methods: : SRN was examined with ophthalmoscopy, fundus photography, fluorescent angiography, and histology at the ages of 2 weeks through 12 months. Various antibodies including markers for activated macrophage, CD11b, and fibroblast, vimentin, were utilized for immunocytochemistry on retina of age–matched wildtype and vldlr–/– mice.

Results: : We found that SRN in vldlr–/– retina began to grow at around 3 weeks, peaked at 4 months, and started to decrease at 8 months of age. Findings from fluorescein angiography were consistent with these observations. The fluorescent leakage was hardly seen at 12 months of age when the fundus was full of scattered, depigmented areas. Histological examination revealed that numerous SRNs were still present at older age; however vascular lumen was often absent, and the RPE layer was disrupted around the SRN sites with significant retinal–choroidal anastomoses. Vimentin immunopositive fibroblasts were identified at SRN sites at 3, 6, 8 and 12 months of age with prominent staining in older animals. Activated macrophages were detected at SRN sites at 5 weeks of age, but not at 12 months of age.

Conclusions: : These results show that fibrosis develops with SRN in vldlr–/– mouse and progresses with age, and the reduced fluorescent leakage in old age is probably due to fibrotic transformation of SRN. Macrophage activation may be involved in the development of SRN in early age of the vldlr–/– mice.

Keywords: retinal neovascularization 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×