May 2006
Volume 47, Issue 13
ARVO Annual Meeting Abstract  |   May 2006
Regulation of Mammalian Retinal Catecholamine Levels by 8–IsoPGF2 and 8–IsoPGE2
Author Affiliations & Notes
  • C.A. Opere
    Pharmacy, Creighton, Omaha, NE
  • M. Zhao
    Pharmacy, Creighton, Omaha, NE
  • H. Liu
    Pharmacy, Creighton, Omaha, NE
  • C.J. Destache
    Pharmacy, Creighton, Omaha, NE
  • R. Zeyssig
    College of Pharmacy, University of Houston, Houston, TX
  • E.M. Monjok
    College of Pharmacy, University of Houston, Houston, TX
  • G.E. Kouamou
    College of Pharmacy, University of Houston, Houston, TX
  • S.E. Ohia
    College of Pharmacy, University of Houston, Houston, TX
  • Footnotes
    Commercial Relationships  C.A. Opere, None; M. Zhao, None; H. Liu, None; C.J. Destache, None; R. Zeyssig, None; E.M. Monjok, None; G.E. Kouamou, None; S.E. Ohia, None.
  • Footnotes
    Support  NIH grant EY – 013967
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 2613. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      C.A. Opere, M. Zhao, H. Liu, C.J. Destache, R. Zeyssig, E.M. Monjok, G.E. Kouamou, S.E. Ohia; Regulation of Mammalian Retinal Catecholamine Levels by 8–IsoPGF2 and 8–IsoPGE2 . Invest. Ophthalmol. Vis. Sci. 2006;47(13):2613.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Introduction: : We have evidence that isoprostanes can modulate the release of excitatory amino acid neurotransmitters from isolated bovine retinae (Opere et al., Neurochem. Res.30:2005).

Purpose: : (1) To determine the effect of 8–iso–prostaglandin (PG)F and 8–isoPGE2 on catecholamine levels in bovine retinae, ex vivo and (2) examine the effect of 8–isoPGE2 on catecholamine concentrations in porcine retinae, in vitro.

Methods: : Isolated bovine eyeballs were injected with isoprostane or vehicle intravitreally. After 30 mins of incubation, retina and vitreous humor were isolated and prepared for HPLC–ED studies. For in vitro studies, isolated porcine neural retina was incubated in Krebs solution containing different concentrations of 8–isoPGE2 (1 – 100 µM) for 15 minutes. After incubation, catecholamines in tissue homogenates and media were extracted with 0.2 N HClO4 and assayed by HPLC–ED.

Results: : In the concentration range, 1 nM to 10µM, intravitreally administered 8–isoPGF increased dopamine and norepinephrine levels in bovine retinae, ex vivo. For instance, 8–isoPGF (1 nM) increased dopamine and norepinephrine concentrations by 52 % (p<0.05) and 75 % (p<0.01), respectively. At 10 µM, 8–isoPGF also increased the levels of catecholamine metabolites, 3,4–dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) by 20% and 50 %, respectively. 8–isoPGE2 (0.1 and 10 µM) increased dopamine levels in bovine retinae by 68.75% (p<0.05) and 80.30% (p<0.01), respectively. In contrast, 8–isoPGE2 (0.1 µM) decreased the levels of DOPAC by 50%. In isolated porcine retinae, 8–isoPGE2 (1 – 100 µM) decreased norepinephrine levels but had no significant (P > 0.05) action on dopamine concentrations.

Conclusions: : We conclude that isoprostanes can modulate catecholamine concentrations in mammalian retinae both ex vivo and in vitro. There is species difference in the response of the retina to 8–isoPGE2.

Keywords: neurotransmitters/neurotransmitter systems • retina • dopamine 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.