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E. Tsuiki, A. Fujita, Y. Ohsaki, J. Cheng, T. Irie, H. Senoo, K. Mishima, T. Kitaoka, T. Fujimoto; All–Trans–Retinol Induces Rapid Recruitment of Tip47 to Lipid Droplets in the Retinal Pigment Epithelium . Invest. Ophthalmol. Vis. Sci. 2006;47(13):2856.
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To determine the existence and the distribution of lipid droplets (LD) and LD proteins in the retinal pigment epithelium (RPE). Furthermore to investigate the effect of light stimulation on LD and LD proteins in the RPE, and to reveal the underlying molecular mechanism.
We used ARPE–19 cells and the RPE of ddy mice. LDs and LD proteins, i.e., adipocyte differentiation–related protein (ADRP), TIP47, and Rab18, were examined by immunohistochemistry. Mouse eyes exposed to flashlight and ARPE–19 cells treated with all–trans–retinol were labeled for immunofluorescence microscopy and the labeling intensity was quantified by image analysis.
ADRP and TIP47 were expressed in the RPE. Flashlight on dark–adapted mouse eyes and all–trans–retinol on ARPE–19 cells caused rapid translocation of TIP47 from the cytosol to LDs, whereas ADRP distributed constitutively in LDs. Western blotting showed that TIP47 expression in RPE did not change by supplementation of all–trans–retinol.
It was revealed that TIP47 in RPE accumulated to LDs in response to all–trans–retinol generated by photobleaching. We speculated that this dynamic translocation of TIP47 is functionally related to the retinoid cycle in the RPE.
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