May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Evaluation of Changes in Retinal Nerve Fiber Layer of Experimental Glaucoma Rat Eyes by Optic Coherence Tomography in vivo
Author Affiliations & Notes
  • D. Liu
    West China Eye Center, West China Hospital, Chengdu, China
  • X. Chen
    West China Eye Center, West China Hospital, Chengdu, China
  • N. Li
    West China Eye Center, West China Hospital, Chengdu, China
  • Y. Liu
    West China Eye Center, West China Hospital, Chengdu, China
  • R. Pan
    West China Eye Center, West China Hospital, Chengdu, China
  • M. Li
    West China Eye Center, West China Hospital, Chengdu, China
  • Footnotes
    Commercial Relationships  D. Liu, None; X. Chen, None; N. Li, None; Y. Liu, None; R. Pan, None; M. Li, None.
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 3372. doi:
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      D. Liu, X. Chen, N. Li, Y. Liu, R. Pan, M. Li; Evaluation of Changes in Retinal Nerve Fiber Layer of Experimental Glaucoma Rat Eyes by Optic Coherence Tomography in vivo . Invest. Ophthalmol. Vis. Sci. 2006;47(13):3372.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To evaluate the changes of the retinal nerve fiber layer (RNFL) in the chronic glaucoma rats using optic coherence tomography (OCT) and the histological examination.

Methods: : Chronic elevation in intraocular pressure (IOP) was induced unilaterally by laser trabecular meshwork photocoagulation in 48 female Wister rats, the opposite eye served as a control. The IOP was measured before laser photocoagulation and weekly thereafter, with Tono–Pen Tonometer. OCT was performed in the 3rd, 6 th and 9 th week, at the same time, IOP was evaluated in both eyes. The OCT images were analyzed for retinal nerve fiber layer. Eyes were nucleated and retina was examined histologically at the 3rd, 6 th and 9 th weeks, respectively.

Results: : IOP was elevated chronically and moderately in laser photocoagulation eyes. The mean IOP was 25.56 ± 4.32 mmHg, 26.64±4.64 mmHg and 27.64±4.28 mmHg in laser treated eyes and 12.75±3.78 mmHg, 13.12±3.64 mmHg and 12.98±3.59 mmHg in the control eyes at the 3rd, 6th and 9th week, respectively. The RNFL thickness, measured by OCT, was 67.39±5.91µm, 53.42±5.64µm and 44.35±5.76µ in laser treated eyes and 76.23±6.78µm, 78.64±6.15µm and 77.64±6.63µm in the control eye at the 3rd, 6th and 9th week respectively. Histological examination showed that the thickness of the retinal nerve fiber layer was 64.38±6.54µm, 51.47±6.41µm and 42.10±6.10µm in the glaucoma eyes and 80.32±5.87µm, 79.69±5.69µm and 80.78±5.84µm in the control eyes at the 3rd, 6th and 9th week respectively. There was a linear correlation between the RNFL thickness measured by OCT and by histological method in the chronic glaucomatous rat model (r=0.932, p<0.001).

Conclusions: : An experimental rat glaucoma model was established successfully by laser trabecular photocoagulation. In the hypertensive eyes, the retinal nerve fiber layer thinned with the passage of time. OCT appeared to be a reliable method to evaluate the changes of the RNFL thickness in vivo.

Keywords: imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • intraocular pressure • nerve fiber layer 
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