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O.P. Kocaoglu, S.R. Uhlhorn, E. Hernandez, R. Will, J.M. Parel, V. Porciatti, F. Manns; In vivo Retinal Imaging of the DBA2/J Glaucoma Mouse Model Using Optical Coherence Tomography . Invest. Ophthalmol. Vis. Sci. 2006;47(13):3374.
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© ARVO (1962-2015); The Authors (2016-present)
To quantify retinal thickness changes of the DBA2/J mouse model of glaucoma in vivo using optical coherence tomography (OCT).
A custom–made mouse retinal imaging system consisting of a bench–top high–resolution OCT system and a slit–lamp based delivery head was developed. The system allows simultaneous non–contact recording of video fundus and cross–sectional OCT images of the same mouse at different time points. Retinal images were recorded in 2 and 14 months old anesthetized DBA/2J mice. After anesthesia, the mice were placed in a cylindrical holder and mounted on a positioner in front of the slit–lamp. The position and alignment of the mice were adjusted until a fundus image was observed. Using the video fundus image for guidance, the OCT spot was then moved to the area of interest using a computer–controlled positioning system and OCT images were acquired. Total retinal thickness was measured from the OCT images at a distance of 2 optic nerve head diameters from the center of the optic nerve. Variability of thickness measurements in mice of the same age measured in two different imaging sessions and differences between 2 and 14 month old mice were quantified.
The average ratio of inner and outer thickness in 2 and 14 month old mice was 0.814±0.085, and 0.683±0.104, which indicates that the thickness reduction mainly stems from changes in the inner retina. The variability of the thickness measurement in the same eye between sessions was 5.8%. The OCT image quality of the 14 month old mice was not as high as in the 2 month old mice because of a loss of optical quality of the eye in the older mice.
The study demonstrates the potential of combined fundus and OCT imaging for in vivo longitudinal studies of disease progression in the DBA2/J mouse model of glaucoma.
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