May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Human Cornea and Aqueous Humor Concentrations of Moxifloxacin and Gatifloxacin Following Topical Ocular Dosing With VIGAMOX® Solution or ZYMAR®*
Author Affiliations & Notes
  • E.J. Holland
    Director, Cincinnati Eye Institute, Cincinnati, OH
  • S. Lane
    Associated Eye Care, Stillwater, OH
  • T. Kim
    Duke University Eye Center, Durham, NC
  • M. Raizman
    Ophthalmic Consultants of Boston, Boston, MA
  • S. Dunn
    Michigan Cornea Consultants, Southfield, MO
  • A. Martin
    Alcon Research LTD, Fort Worth, TX
  • D. Jasheway
    Alcon Research LTD, Fort Worth, TX
  • H. Katz
    Krieger Eye Institute, Baltimore, MD
  • S. Robertson
    Alcon Research LTD, Fort Worth, TX
  • D. Dahlin
    Alcon Research LTD, Fort Worth, TX
  • Footnotes
    Commercial Relationships  E.J. Holland, Alcon, F; S. Lane, Alcon, F; T. Kim, Alcon, F; M. Raizman, Alcon, F; S. Dunn, Alcon, F; A. Martin, Alcon, E; D. Jasheway, Alcon, E; H. Katz, Alcon, F; Alcon, C; S. Robertson, Alcon, E; D. Dahlin, Alcon, E.
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 3577. doi:
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      E.J. Holland, S. Lane, T. Kim, M. Raizman, S. Dunn, A. Martin, D. Jasheway, H. Katz, S. Robertson, D. Dahlin; Human Cornea and Aqueous Humor Concentrations of Moxifloxacin and Gatifloxacin Following Topical Ocular Dosing With VIGAMOX® Solution or ZYMAR®* . Invest. Ophthalmol. Vis. Sci. 2006;47(13):3577.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To evaluate the corneal penetration of moxifloxacin (moxi) and gatifloxacin (gati) following topical ocular administration of VIGAMOX or ZYMAR by measuring the concentrations of moxi and gati in corneal tissue and aqueous humor (AH) of patients undergoing penetrating keratoplasty (PK).

Methods: : In a controlled, randomized, open–label, multiple–dose study, corneas from 48 patients undergoing PK were analyzed following administration of either VIGAMOX (moxifloxacin 0.5%) or ZYMAR (gatifloxacin 0.3%). Prior to surgery, the study eye was treated with 2 doses of test article (1 drop per dose) given 5 minutes apart, with the last dose at 0.25, 0.5, 1, or 2 hours prior to AH and cornea collection. Following AH sampling and excision of the corneal button, samples were placed on dry ice and shipped for analysis. Corneas were dissected into epithelium, stroma and endothelium and were assayed using a validated simultaneous HPLC–fluorescence method.

Results: : The mean peak epithelial level of moxi (81.2 µg/g) was approximately 7–fold higher than that for gati (12.3 µg/g). A 3–fold higher peak stromal level was observed for moxi (48.5 µg/g) versus gati (15.7 µg/g). A mean peak endothelial level of moxi (76.1 µg/g) was approximately 10–fold higher than that for gati (7.3 µg/g). Consistent with prior clinical studies, the mean peak AH level of moxi was approximately 3–fold higher than gati. Peak levels of moxi were considerably higher relative to MIC values for the most common endophthalmitis and keratitis pathogens (including atypical mycobacteria).

Conclusions: : The higher corneal and AH concentrations and greater antimicrobial activity of moxi demonstrate that VIGAMOX provides enhanced activity against common ocular pathogens associated with endophthalmitis and keratitis. Licensed to Alcon, Inc. by Bayer AG *Zymar is a reg. tm of Allergan, Inc.

Keywords: antibiotics/antifungals/antiparasitics 
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