May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
The Effects of TNF–Alpha and Dexamethasone on Cultured Hyalocytes
Author Affiliations & Notes
  • Y. Hata
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • M. Miura
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Y. Sassa
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • K. Kano
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • T. Kita
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • S. Nakao
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • T. Ishibashi
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Footnotes
    Commercial Relationships  Y. Hata, None; M. Miura, None; Y. Sassa, None; K. Kano, None; T. Kita, None; S. Nakao, None; T. Ishibashi, None.
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 3825. doi:
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      Y. Hata, M. Miura, Y. Sassa, K. Kano, T. Kita, S. Nakao, T. Ishibashi; The Effects of TNF–Alpha and Dexamethasone on Cultured Hyalocytes . Invest. Ophthalmol. Vis. Sci. 2006;47(13):3825.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Tumor necrosis factor–α (TNF–α) is one of the major inflammatory cytokines which is supposed to be involved in the pathogenesis of various vitreoretinal diseases. We investigated the effect of TNF–α and Dexamethasone on the functional property of cultured hyalocytes.

Methods: : The hyalocytes were isolated from bovine vitreous as we previously reported. TNF–α–dependent proliferative activity of the hyalocytes was analyzed by detecting the phosphorylation status of p44/p42 MAPK, [3H]–thymidine uptake assay and cell counting. Boyden chamber assay was performed to evaluate the migration activity of the cells. An in vitro type I collagen gel contraction assay was also performed. Furthermore, we examined the effects of dexamethasone on the functional property of hyalocytes in the presence of TNF–α.

Results: : The hyalocytes were isolated from bovine vitreous as we previously reported. TNF–α–dependent proliferative activity of the hyalocytes was analyzed by detecting the phosphorylation status of p44/p42 MAPK, [3H]–thymidine uptake assay and cell counting. Boyden chamber assay was performed to evaluate the migration activity of the cells. An in vitro type I collagen gel contraction assay was also performed. Furthermore, we examined the effects of dexamethasone on the functional property of hyalocytes in the presence of TNF–α.

Conclusions: : TNF–α might be involved in the pathogenesis of vitreoretinal diseases by inducing proliferative membrane formation by hyalocytes at least in part. Dexamethasone seems to be beneficial for inhibiting membrane formation but might be harmful for cicatrical contraction of the membranous tissue.

Keywords: proliferative vitreoretinopathy • vitreous 
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