May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
A Comparative Study of Effects of Topoisomerase Inhibitors on Retinal Pigment Epithelial Cells
Author Affiliations & Notes
  • H.–K. Kuo
    Ophthalmology, Chang Gung Memorial Hospital, Kaohsiung, Kaohsiung Hsien, Taiwan Republic of China
  • P.–C. Wu
    Ophthalmology, Chang Gung Memorial Hospital, Kaohsiung, Kaohsiung Hsien, Taiwan Republic of China
  • Y.–H. Chen
    Ophthalmology, Chang Gung Memorial Hospital, Kaohsiung, Kaohsiung Hsien, Taiwan Republic of China
  • Y.–C. Wu
    Ophthalmology, Chang Gung Memorial Hospital, Kaohsiung, Kaohsiung Hsien, Taiwan Republic of China
  • Footnotes
    Commercial Relationships  H. Kuo, None; P. Wu, None; Y. Chen, None; Y. Wu, None.
  • Footnotes
    Support  Taiwan NSC grant 94–2314–B–182A
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 3826. doi:
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      H.–K. Kuo, P.–C. Wu, Y.–H. Chen, Y.–C. Wu; A Comparative Study of Effects of Topoisomerase Inhibitors on Retinal Pigment Epithelial Cells . Invest. Ophthalmol. Vis. Sci. 2006;47(13):3826.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The inhibition of RPE proliferation by chemotherapeutic agents remains a primary target in the treatment of PVR. In this study, we investigate the effect of some commercially available topoisomerase inhibitors on the RPE cells.

Methods: : Study medications included topotecan, etoposide, doxorubicin, and daunorubicin at various concentrations (100, 50, 30, 10, 1, 0.1, 0.01 ug/ml). Rabbit RPE cells were used. After 48 and 96 hours, MTT assay and cell counting were used to determine cell survival and proliferation rate.

Results: : Four drugs all inhibited proliferation of RPE cells in a dose–dependent manner in vitro. The actual numbers of cells correlated well with the results of the MTT test. The IC50 is defined as the concentration of a drug that produces a 50% reduction in cell number. The IC50 of topotecan, etoposide, doxorubicin, and daunorubicin was 0.1–1, 1–10, <0.1, and <0.1 ug/ml respectively at 48hours. The slope of dose–response curve was slowly declined for topotecan and etoposide, but very sharp for doxorubicin and daunorubicin.

Conclusions: : Topotecan and etoposide have both effective and safe property as RPE cells inhibitors.

Keywords: proliferative vitreoretinopathy • retinal pigment epithelium 
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