Abstract
Purpose: :
To investigate the 50 % lethality of monochromatic ultraviolet (UV) radiation on porcine lens epithelial cells (LECs).
Methods: :
Porcine LECs were isolated and were grown in DMEM–F12 medium supplemented with 10% fetal bovine serum (FBS) and antibiotics. LECs were grown to confluence in 24–well plate, and the medium was changed to DMEM–F12 with 0.2% FBS. This 0.2% FBS medium for 24 hr at 37 degrees and 5% CO2 incubation induced G(0) cell cycle arrest. For each set of experiments, the culture plate was wrapped by a special black cover which had one open place for one well for irradiation. Each well was irradiated by 280 nm (0.5, 1.0, 1.5, 2.0, 2.5 mJ/cm2), 291 nm (2, 4, 6, 8, 10 mJ/cm2), 301 nm (5, 10, 15, 20, 25 mJ/cm2), 309 nm (100, 200, 300, 400, 500 mJ/cm2) UV light. Twenty four hr after the irradiation, each well took photograph by digital camera, counted living cell numbers, and calculated 50% lethality of monochromatic UV irradiation dose.
Results: :
50% lethality of monochromatic UV irradiation dose were 1.39 mW/cm2 at 280 nm, 3.22 mW/cm2 at 291 nm, 13.9 mW/cm2 at 301 nm, and 232 mW/cm2 at 309 nm, respectively.
Conclusions: :
These results were close relation of 50% lethality of UV irradiation dose on human lens epithelial cells (Okuno T., et al, 2003KMU, Workshop on Ocular Changes Induced by Electromagnetic Waves, Kanazawa, Japan).
Keywords: radiation damage: light/UV • apoptosis/cell death • oxidation/oxidative or free radical damage