May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Effects Of Dexamethasone In The Lens
Author Affiliations & Notes
  • A. Petersen
    Goteborg University, Goteborg, Sweden
    Inst. of Anatomy & Cell Biology,
  • M. Zetterberg
    Goteborg University, Goteborg, Sweden
    Section of Ophthalmology, Inst. of Clinical Neuroscience,
  • T. Carlsson
    Goteborg University, Goteborg, Sweden
    Inst. of Anatomy & Cell Biology,
  • J. Sjöstrand
    Goteborg University, Goteborg, Sweden
    Section of Ophthalmology, Inst. of Clinical Neuroscience,
  • J.–O. Karlsson
    Goteborg University, Goteborg, Sweden
    Inst. of Anatomy & Cell Biology,
  • Footnotes
    Commercial Relationships  A. Petersen, None; M. Zetterberg, None; T. Carlsson, None; J. Sjöstrand, None; J. Karlsson, None.
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 4110. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      A. Petersen, M. Zetterberg, T. Carlsson, J. Sjöstrand, J.–O. Karlsson; Effects Of Dexamethasone In The Lens . Invest. Ophthalmol. Vis. Sci. 2006;47(13):4110.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : The aim of the study was to investigate effects of glucocorticoids in the lens.

Methods: : Lens epithelial cells (HLEC) were exposed to dexamethasone for 24 hours. Cells were assayed for changes in superoxide production using dihydroethidium (HET), for alterations in peroxide production using DCFH–DA or for GSH variations using monochlorobimane (MCB). Apoptosis was determined by Caspase–3 assay and by nuclear morphology of Hoechst stained cells. Mitochondria depolarisation was measured using the potential–sensitive colour JC–1. Morphology was examined by transmission electron microscopy (TEM).

Results: : Apoptosis were increased in HLEC exposed to 1, 10, 100 and 1000 µM dexamethasone as revealed by nuclear morphology studies. Caspase–3 activity was increased at 100 and 1000 µM dexamethasone. No effect on GSH, superoxide or peroxide production by dexamethasone was present. High concentrations of dexamethasone (1000 µM) depolarised the mitochondria. TEM showed multilayering of cells, mitochondrial changes and accumulation of membrane delimited electron dense material.

Conclusions: : The mechanism underlying dexamethasone induced apoptosis and morphological changes in HLEC are probably not due to oxidative effects.

Keywords: crystalline lens • corticosteroids • apoptosis/cell death 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×