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J. Koh, Y. Yang; The Effects of Glucose Concentrations on Reactive Oxygen Products in Human Lens Epithelial Cells . Invest. Ophthalmol. Vis. Sci. 2006;47(13):4111.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the effects of glucose concentrations on formation of reactive oxygen products and cellular injury in human lens epithelial(HLE) cells.
Human lens epithelial(HLE) cells were cultured with high glucose(200mg/100ml, 300mg/100ml, 400mg/100ml) and normal glucose(100mg/100ml) contained MEM. The amount of reactive oxygen products formation were measured by using dihydroethidium(DHE). Paraquat–induced cytotoxicity was determined by 3–[4,5–dimethylthiazol–2–yl]–2,5 –diphenyl tetrazolium bromide (MTT) method.
Reactive oxygen products of human lens epithelial cells were increased 110%, 250%. 630% in high glucose(200mg/100ml, 300mg/100ml, 400mg/100ml) media compared to normal glucose(100mg/100ml) media. The paraquat–induced cells toxicity was increased by high glucose.
High glucose increased formation of reactive oxygen products in human lens epithelial cells. These results suggest that high glucose can induce cataract formation in diabetic patient.
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