May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Atorvastatin Inhibits Laser–Induced Experimental Choroidal Neovascularization Through Downregulation of Vascular Endothelial Growth Factor
Author Affiliations & Notes
  • K. Yamada
    Ophthalmology and Visual Science, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan
  • E. Sakurai
    Ophthalmology and Visual Science, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan
  • S. Yamasaki
    Ophthalmology and Visual Science, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan
  • M. Itaya
    Ophthalmology and Visual Science, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan
  • Y. Ogura
    Ophthalmology and Visual Science, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan
  • Footnotes
    Commercial Relationships  K. Yamada, None; E. Sakurai, None; S. Yamasaki, None; M. Itaya, None; Y. Ogura, None.
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 4154. doi:
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      K. Yamada, E. Sakurai, S. Yamasaki, M. Itaya, Y. Ogura; Atorvastatin Inhibits Laser–Induced Experimental Choroidal Neovascularization Through Downregulation of Vascular Endothelial Growth Factor . Invest. Ophthalmol. Vis. Sci. 2006;47(13):4154.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate the effect of atorvastatin, HMG CoA reductase inhibitor, on experimental choroidal neovascularization (CNV) induced by laser photocagulation in mice.

Methods: : CNV was experimentally induced by the laser photocoagulation in normal wild–type (C57Bl/6J) mice. The mice orally received atorvastatin 10 mg/kg/day (AS10 group, n=10) or 20 mg/kg/day (AS20 group, n=10) for 3 days prior to laser application. Development of CNV was studied on the basis of dimension measurements obtained by confocal microscopy 1 week after laser application. VEGF protein levels from RPE/choroid lysates were measured by ELISA at 3 days after photocoagulation. Macrophage infiltration to RPE /choroid was quantitatively studied by flow cytemetry getting with F4/80+ cells.

Results: : Mean CNV area was significantly smaller in the AS10 (non–paired t test, 11249.30±2353.85µm2, p<0.001) and AS20 (13717.13±3357.36µm2, p=0.008) groups compared to control mice (26565.65±3180.67µm2). Mean VEGF levels were significantly reduced in AS10 (62.3±4.7%, p=0.001) and AS20 (52.9±4.5%, p=0.004) groups compared to the controls. The numbers of macrophage infiltrated into the RPE/choroid was also reduced in AS10 (64.2±4.5%, p=0.03) and AS20 (58.5±7.2%, p=0.01) groups.

Conclusions: : Atorvastatin treatment effectively inhibited the laser–induced CNV in mice. It was associated with the downregulation of VEGF and the reduced macrophage infiltration in the choroids.

Keywords: age-related macular degeneration • choroid: neovascularization • cytokines/chemokines 
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