May 2006
Volume 47, Issue 13
ARVO Annual Meeting Abstract  |   May 2006
CLIC4–Mediated RPE Morphogenesis and RPE–Photoreceptor Interaction
Author Affiliations & Notes
  • C.–H. Sung
    Ophth Cell Biololoy Anatomy, Weill Med Coll of Cornell Univ, New York, NY
  • J.–Z. Chuang
    Ophth Cell Biololoy Anatomy, Weill Med Coll of Cornell Univ, New York, NY
  • Footnotes
    Commercial Relationships  C. Sung, None; J. Chuang, None.
  • Footnotes
    Support  NIH Grant EY11307, RPB, and Steinbach Research Foundation
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 4226. doi:
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      C.–H. Sung, J.–Z. Chuang; CLIC4–Mediated RPE Morphogenesis and RPE–Photoreceptor Interaction . Invest. Ophthalmol. Vis. Sci. 2006;47(13):4226.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : The retinal pigment epithelium (RPE) is a multifunctional and indispensable component of the vertebrate retina. Its asymmetry, specialized membrane structures, and membrane motility, which are essential for many of its functions, rely heavily on a highly ordered cytoskeletons. At present, relatively little is known about the machinery and the molecular mechanism regulating cytoskeleton–mediated RPE functions. We found CLIC4, a recently identified actin–associated protein, was abundantly expressed in apical RPE microvilli. The purpose of these studies is to determine the functions of CLIC4 in RPE in vivo.

Methods: : We performed CLIC4 silencing by transfecting siRNA (small interfering RNA) against CLIC4 into RPE of rodent eyes. RPE sheets or retina sections containing CLIC4–suppressed RPE were examined histologically and immunohistochemically.

Results: : The CLIC4 suppressed RPE cells developed several morphological changes including microvillus shortening, cytoskeleton re–arrangement, and cell–cell contact breakdown. Moreover, these animals developed profound retinal detachment and photoreceptor dystrophy.

Conclusions: : CLIC4 is a key molecule involved in the morphogenesis of RPE and its interdigit interaction with neural photoreceptors.

Keywords: retinal pigment epithelium • retinal adhesion • cell membrane/membrane specializations 

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