Purchase this article with an account.
L. Cimino, L. Pattacini, B. Casali, A. Magnani, L. Cappuccini, C. Salvarani; Determination Of Reelin Levels In Aqueous Humor Of Patients With Uveitis. . Invest. Ophthalmol. Vis. Sci. 2006;47(13):4518.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Reelin is a large secreted serine protease involved in neuron migration during laminar structure formation. Reelin is expressed in tissues other than central nervous system, such as olfactory bulb, retina and spinal cord. In this study we evaluated the reelin levels in patients with different forms of uveitis.
the patients were seen at the Ocular Immunology Unit in the Hospital of Reggio Emilia. We selected a control group of 10 patients, 13 patients with Fuch’s uveitis, 9 patients with infectious uveitis and 8 patients with autoimmune systemic diseases. The aqueous humor was obtained by paracentesis. Reelin was detected by a SDS–PAGE separation followed by western blotting performed with a specific antibody against the N–terminal sequence of the protein. Densitometric evaluation was performed using Scion Image software.
Reelin was detected in all the samples analyzed by western blotting. For each sample, we determined the ratio between the densitometric value of the 150KD band of reelin and the same band obtained with the recombinant reelin. We compared the values of ratio for the four selected groups. The mean value for the control group was 0.69± 0.33, 1.20±0.40 for Fuchs patients, 1.23±0.77 for patients with infectious uveitis and 0.93±0.34 for patients with systemic pathologies. There were significant differences between the control and Fuch’s group (p=0.047), and the control and the infectious uveitis group (p=0.039), but not between control and systemic diseases group (p=0.071).
This data suggest that reelin is present besides the Central Nervous System in aqueous humor and that its levels are increased in inflammatory and infectious uveitis. We can hypothesise that the proteolytic activity of reelin on adhesion molecules of the extracellular matrix could explain its involvement in the inflammatory/infectious process.
This PDF is available to Subscribers Only