May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Chemotactic And Chemokinetic Properties Of Topical Ophthalmic Therapeutic Agents In Uveitis Patients
M.E. Zajdenweber; E. Antecka; J. Deschenes; K. Godeiro; P. Ozdal; M.G. Baines; M.N. Burnier
Author Affiliations & Notes
  • M.E. Zajdenweber
    McGill University, Montreal, PQ, Canada
    Henry C. Witelson Ocular Pathology Lab,
    Ophthalmology, Federal University of Sao Paulo, Sao Paulo, Brazil
  • E. Antecka
    McGill University, Montreal, PQ, Canada
    Henry C. Witelson Ocular Pathology Lab,
  • J. Deschenes
    McGill University, Montreal, PQ, Canada
    Ophthalmology,
  • K. Godeiro
    McGill University, Montreal, PQ, Canada
    Henry C. Witelson Ocular Pathology Lab,
  • P. Ozdal
    McGill University, Montreal, PQ, Canada
    Henry C. Witelson Ocular Pathology Lab,
  • M.G. Baines
    McGill University, Montreal, PQ, Canada
    Microbiology and Immunology,
  • M.N. Burnier
    McGill University, Montreal, PQ, Canada
    Henry C. Witelson Ocular Pathology Lab,
    Ophthalmology,
  • Footnotes
    Commercial Relationships  M.E. Zajdenweber, None; E. Antecka, None; J. Deschenes, None; K. Godeiro, None; P. Ozdal, None; M.G. Baines, None; M.N. Burnier, None.
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 4520. doi:
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      M.E. Zajdenweber, E. Antecka, J. Deschenes, K. Godeiro, P. Ozdal, M.G. Baines, M.N. Burnier; Chemotactic And Chemokinetic Properties Of Topical Ophthalmic Therapeutic Agents In Uveitis Patients . Invest. Ophthalmol. Vis. Sci. 2006;47(13):4520.

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Abstract

Purpose: : Leukocytes is part of inflammation .The purpose of this study is to investigate the effect on the migration of leukocytes by ophthalmic medications that are commonly used in patients with uveitis.

Methods: : A modified multi–well Boyden chamber was used to study the chemotaxis and chemokinesis properties of 13 commercial ophthalmic medications commonly used in the treatment of uveitis patients. A migration assay was performed in 2 groups: group 1 with 12 healthy individuals and group 2 with 8 uveitis patients. For each group, a blood sample was taken and leukocytes were isolated. Chemotaxis and chemokinesis assay for 13 ophthalmic preparations (Prednisolone® , Flarex® , InflamaseForte 1%® , Maxidex® , Voltaren® , Acular® , Cyclogyl® , Mydriacyl® , Isopto–Homatropine® , Lumigan® , Trusopt® ,Timoptic® , Alphagan® and 2 controls (ZAS as positive control and PBS as negative control) were performed. The paired Student 's T test was used to compare results in each patient group and the independent–sample Student 's T test was used to compare results between patient groups.

Results: : In group1, compared to negative control, Inflamase Forte® produced the greatest leukocyte migration (20.53µ ±5.30, p<0.001) and Acular® produced the least leukocyte migration (–7.63µ ±4.90, p<0.001). In group 2, compared to negative control, Lumigan® produced the greatest leukocyte migration (14.38µ ±4.30, p<0.001) and Isopto–Homatropine® produced the least leukocyte migration (–20.85µ ± 3.90, p<0.001). Comparisons between the 2 groups showed that all preparations in group one, except Lumigan® and Alphagan® , to be significantly more chemotactic and chemokinetic (p<0.05).

Conclusions: : Most of the preparations tested caused significant differences in chemotaxis and chemokinesis. Leukocytes isolated from normal controls had significantly higher migration rates than those isolated from uveitis patients. This data may be used to tailor treatment strategies for uveitis patients.

Keywords: inflammation • drug toxicity/drug effects • pharmacology 
© 2006, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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