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M.E. Zajdenweber, E. Antecka, J. Deschenes, K. Godeiro, P. Ozdal, M.G. Baines, M.N. Burnier; Chemotactic And Chemokinetic Properties Of Topical Ophthalmic Therapeutic Agents In Uveitis Patients . Invest. Ophthalmol. Vis. Sci. 2006;47(13):4520.
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© ARVO (1962-2015); The Authors (2016-present)
Leukocytes is part of inflammation .The purpose of this study is to investigate the effect on the migration of leukocytes by ophthalmic medications that are commonly used in patients with uveitis.
A modified multi–well Boyden chamber was used to study the chemotaxis and chemokinesis properties of 13 commercial ophthalmic medications commonly used in the treatment of uveitis patients. A migration assay was performed in 2 groups: group 1 with 12 healthy individuals and group 2 with 8 uveitis patients. For each group, a blood sample was taken and leukocytes were isolated. Chemotaxis and chemokinesis assay for 13 ophthalmic preparations (Prednisolone® , Flarex® , InflamaseForte 1%® , Maxidex® , Voltaren® , Acular® , Cyclogyl® , Mydriacyl® , Isopto–Homatropine® , Lumigan® , Trusopt® ,Timoptic® , Alphagan® and 2 controls (ZAS as positive control and PBS as negative control) were performed. The paired Student 's T test was used to compare results in each patient group and the independent–sample Student 's T test was used to compare results between patient groups.
In group1, compared to negative control, Inflamase Forte® produced the greatest leukocyte migration (20.53µ ±5.30, p<0.001) and Acular® produced the least leukocyte migration (–7.63µ ±4.90, p<0.001). In group 2, compared to negative control, Lumigan® produced the greatest leukocyte migration (14.38µ ±4.30, p<0.001) and Isopto–Homatropine® produced the least leukocyte migration (–20.85µ ± 3.90, p<0.001). Comparisons between the 2 groups showed that all preparations in group one, except Lumigan® and Alphagan® , to be significantly more chemotactic and chemokinetic (p<0.05).
Most of the preparations tested caused significant differences in chemotaxis and chemokinesis. Leukocytes isolated from normal controls had significantly higher migration rates than those isolated from uveitis patients. This data may be used to tailor treatment strategies for uveitis patients.
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