May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Specific Chemokine Gene Expression in the Retina and T Cells Related to the Development of Experimental Autoimmune Uveoretinitis Compared With Other Immunized Mouse Models
Author Affiliations & Notes
  • M. Takeuchi
    Tokyo Medical University, Tokyo, Japan
    Ophthalmology,
  • A. Takeuchi
    Tokyo Medical University, Tokyo, Japan
    Ophthalmology,
  • Y. Usui
    Tokyo Medical University, Tokyo, Japan
    Ophthalmology,
  • T. Hattori
    Tokyo Medical University, Tokyo, Japan
    Ophthalmology,
  • Y. Okunuki
    Tokyo Medical University, Tokyo, Japan
    Ophthalmology,
  • K. Yoshida
    Tokyo Medical University, Tokyo, Japan
    Pathology,
  • M. Kuroda
    Tokyo Medical University, Tokyo, Japan
    Pathology,
  • M. Usui
    Tokyo Medical University, Tokyo, Japan
    Ophthalmology,
  • Footnotes
    Commercial Relationships  M. Takeuchi, None; A. Takeuchi, None; Y. Usui, None; T. Hattori, None; Y. Okunuki, None; K. Yoshida, None; M. Kuroda, None; M. Usui, None.
  • Footnotes
    Support  Grant–in–Aid 16591769 for Scientific Research from the Japan Society for the Promotion of Science
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 4536. doi:
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    • Get Citation

      M. Takeuchi, A. Takeuchi, Y. Usui, T. Hattori, Y. Okunuki, K. Yoshida, M. Kuroda, M. Usui; Specific Chemokine Gene Expression in the Retina and T Cells Related to the Development of Experimental Autoimmune Uveoretinitis Compared With Other Immunized Mouse Models . Invest. Ophthalmol. Vis. Sci. 2006;47(13):4536.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Development of organ–specific autoimmune diseases is thought to be regulated by signals transmitted between local tissue–specific cells and circulating immune cells. In this study, we attempted to identify chemoattractive signals specific for the development of experimental autoimmune uveoretinitis (EAU) by comparing total gene expression between mice immunized with human IRBP (interphotoreceptor retinoid–binding protein) peptide 1–20 (hIRBP–p) and mice immunized with other peptides.

Methods: : C57BL/6 mice were immunized with 200 ºg of BSA, peptide 35–55 of myelin oligodendrocyte protein (MOG–p), or hIRBP–p emulsified in complete Freund's adjuvant. On day 14, retinas and splenic T cells were collected, and total RNAs were extracted. The expression profiles of approximately 20,000 potentially relevant genes were analyzed using pooled RNA samples within individual groups and the Codelink Bioarray system. The data were evaluated by the gene expression ratio of each experimental group versus that of non–immunized control group.

Results: : In the retina of mice immunized with hIRBP–p that induce EAU, genes of CCL5, CXCL9, and CXCL10 related to Th1 cell recruitment and those of CCL7, CCL8 and CCL17 associated with Th2 cell recruitment were preferentially expressed compared with other chemokine genes. Although these chemokine genes were also expressed in the retina of mice immunized with MOG–p that induce optic neuritis in addition to experimental autoimmune encephalomyelitis, the overall level of expression was lower than that in the retina with induced EAU. In T cells, EAU–specific Th1 or Th2 chemokine gene expression was not observed. Interestingly, gene of CCL2, CCL24 or CXCL7 responsible for macrophage and neutrophil recruitment was specifically detected in the retina and T cells of mice with induced EAU and not in the other immunized groups.

Conclusions: : These results suggest that the recruitment of macrophages and neutrophils may preferentially contribute to the development of EAU compared with other immunized animal models.

Keywords: uveitis-clinical/animal model • autoimmune disease • cytokines/chemokines 
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