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Y.–H. Chen, H.–K. Kuo, P.–C. Wu, L.–S. Cheng, M.–H. Tai; Inhibition of Experimental Proliferative Vitreoretinopathy by Gene Therapy With PTEN . Invest. Ophthalmol. Vis. Sci. 2006;47(13):4666.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate the inhibitory activity of PTEN on the formation of experimental PVR
In vitro, the RPE cells were infected with adenovirus encoding PTEN. The cell proliferation was determined at one and two days using MTT assay. The PTEN gene expression was analyzed by Western blot. In vivo, the left eye of pigmented rabbits was injected with 1×105 cells of RPE transfected with PTEN. In contrast, the right eye of rabbits was injected with 1×105 cells of RPE transfected with GFP only.
The MTT analysis revealed the proliferation rate was decreased to the extent of 80% in the Ad–PTEN group compared with the control group. Western blot analysis confirmed the exogenous PTEN and GFP gene were successfully introduced to the RPE cell by the adenoviruses. PVR progression was similar in both groups of rabbits during the 4–week follow–up. At day 28, the mean PVR grade was 2.3 in the experimental group and 1.7 in the control group. Two groups didn’t have the statistical difference about the PVR grading.
The PTEN gene is successfully transinfected to the RPE cell, but the suppressive effect was relative limited. The exogenous PTEN gene did not modify the development of cell–induced PVR.
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