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H. Okamoto, S. Umeda, M.T. Suzuki, K. Terao, T. Nozawa, Y. Yoshikawa, Y. Miyake, T. Iwata; Comparative Proteome Analysis of Macula versus Peripheral Retina in Cynomolgus Monkey . Invest. Ophthalmol. Vis. Sci. 2006;47(13):4866.
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© ARVO (1962-2015); The Authors (2016-present)
Macular diseases including age–related macular degeneration (ARMD) is preferentially affect the macular region of the retina. This may be due to the tissue characteristic and proteins expressed in the macular region. To determine the proteins specifically expressed in the macular, comprehensive proteome analysis of macular versus peripheral retina was performed using by two dimensional (2D) gel separation and mass spectrometer of cynomolgus monkey eyes.
Tissue from macular and peripheral retina including RPE cell and choroidal membrane was obtained from eight normal monkeys. 2D gel electrophoresis was performed in four different pH ranges (pH3–10, pH4–7, pH5–8, and pH7–10) for protein separation. Gel was stained and scanned for spot comparison between macular and peripheral retina. Identification of protein in gel spot was carried out by ion spray LC–MS/MS (LCQ Deca XP plus and Finnigan LTQ, ThermoElectron).
From pH3 to pH10, approximately seven hundred spots were detected on the gel image for macular and peripheral retina samples respectively. Sixty percent of these spots were present in both samples. Spots detected only macular involved arrestin 3, component of mitochondria and gamma–synuclein.
Macular specific proteins from cynomolgus monkey eyes were identified by 2D gel and mass spectrometer. Arrestin 3, component of mitochondria, and gamma–synuclein were identified. These and other proteins expressed in macula will be investigated by immunohistochemical experiments.
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