May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Comparative Proteome Analysis of Macula versus Peripheral Retina in Cynomolgus Monkey
H. Okamoto; S. Umeda; M.T. Suzuki; K. Terao; T. Nozawa; Y. Yoshikawa; Y. Miyake; T. Iwata
Author Affiliations & Notes
  • H. Okamoto
    National Institute of Sensory Organs, National Hospital Organization Tokyo Medical Center, Tokyo, Japan
  • S. Umeda
    National Institute of Sensory Organs, National Hospital Organization Tokyo Medical Center, Tokyo, Japan
  • M.T. Suzuki
    The Corporation for Production and Research of Laboratory Primates, Tukuba, Japan
  • K. Terao
    Tsukuba Primate Medical Research Center, National Institute of Biomedical Innovation, Tukuba, Japan
  • T. Nozawa
    Analytical Instrument Division, AMR Inc., Tokyo, Japan
  • Y. Yoshikawa
    Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
  • Y. Miyake
    National Institute of Sensory Organs, National Hospital Organization Tokyo Medical Center, Tokyo, Japan
  • T. Iwata
    National Institute of Sensory Organs, National Hospital Organization Tokyo Medical Center, Tokyo, Japan
  • Footnotes
    Commercial Relationships  H. Okamoto, None; S. Umeda, None; M.T. Suzuki, None; K. Terao, None; T. Nozawa, AMR Inc., E; Y. Yoshikawa, None; Y. Miyake, None; T. Iwata, None.
  • Footnotes
    Support  Ministry of Health, Labour, and Welfare of Japan
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 4866. doi:
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      H. Okamoto, S. Umeda, M.T. Suzuki, K. Terao, T. Nozawa, Y. Yoshikawa, Y. Miyake, T. Iwata; Comparative Proteome Analysis of Macula versus Peripheral Retina in Cynomolgus Monkey . Invest. Ophthalmol. Vis. Sci. 2006;47(13):4866.

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Abstract

Purpose: : Macular diseases including age–related macular degeneration (ARMD) is preferentially affect the macular region of the retina. This may be due to the tissue characteristic and proteins expressed in the macular region. To determine the proteins specifically expressed in the macular, comprehensive proteome analysis of macular versus peripheral retina was performed using by two dimensional (2D) gel separation and mass spectrometer of cynomolgus monkey eyes.

Methods: : Tissue from macular and peripheral retina including RPE cell and choroidal membrane was obtained from eight normal monkeys. 2D gel electrophoresis was performed in four different pH ranges (pH3–10, pH4–7, pH5–8, and pH7–10) for protein separation. Gel was stained and scanned for spot comparison between macular and peripheral retina. Identification of protein in gel spot was carried out by ion spray LC–MS/MS (LCQ Deca XP plus and Finnigan LTQ, ThermoElectron).

Results: : From pH3 to pH10, approximately seven hundred spots were detected on the gel image for macular and peripheral retina samples respectively. Sixty percent of these spots were present in both samples. Spots detected only macular involved arrestin 3, component of mitochondria and gamma–synuclein.

Conclusions: : Macular specific proteins from cynomolgus monkey eyes were identified by 2D gel and mass spectrometer. Arrestin 3, component of mitochondria, and gamma–synuclein were identified. These and other proteins expressed in macula will be investigated by immunohistochemical experiments.

Keywords: macula/fovea • proteomics • age-related macular degeneration 
© 2006, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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