May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Analysis of Extraocular Behavior of Mucoadhesive Chitosan–Coated Emulsion
M. Yamaguchi; K. Ueda; A. Isowaki; A. Ohtori; H. Takeuchi; N. Ohguro; K. Tojo
Author Affiliations & Notes
  • M. Yamaguchi
    Research Laboratories, SENJU pharmaceutical Co., Ltd., Hyogo, Japan
  • K. Ueda
    Research Laboratories, SENJU pharmaceutical Co., Ltd., Hyogo, Japan
  • A. Isowaki
    Research Laboratories, SENJU pharmaceutical Co., Ltd., Hyogo, Japan
  • A. Ohtori
    Research Laboratories, SENJU pharmaceutical Co., Ltd., Hyogo, Japan
  • H. Takeuchi
    Department of Pharmaceutical Engineering, Gifu Pharmaceutical University, Gifu, Japan
  • N. Ohguro
    Department of Ophthalmology, Osaka University Medical School, Osaka, Japan
  • K. Tojo
    Department of Biochemical Science and Engineering, Kyushu Institute of Technology, Fukuoka, Japan
  • Footnotes
    Commercial Relationships  M. Yamaguchi, None; K. Ueda, None; A. Isowaki, None; A. Ohtori, None; H. Takeuchi, None; N. Ohguro, None; K. Tojo, None.
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 5090. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      Analysis of Extraocular Behavior of Mucoadhesive Chitosan–Coated Emulsion
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      M. Yamaguchi, K. Ueda, A. Isowaki, A. Ohtori, H. Takeuchi, N. Ohguro, K. Tojo; Analysis of Extraocular Behavior of Mucoadhesive Chitosan–Coated Emulsion . Invest. Ophthalmol. Vis. Sci. 2006;47(13):5090.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract

Purpose: : Chitosan–coated ophthalmic emulsions (CE) were reported to improve the bioavailability of drug after instillation due to prolonged residence time of drug in tear by mucoadhesive effect of positively charged chitosan onto conjunctiva. However, pharmacokinetic analysis of CE in tear has not been elucidated yet. In this study, we have evaluated the distribution behavior of CE in extraocular tissues by visual analysis using a confocal laser scanning microscopy. The extraocular profiles for elimination were then analyzed by the side–capacity–parallel–flow (SCPF) model.

Methods: : CE and non–coated emulsions (NE) containing a fluorescent dye were instilled into rabbit eyes. The eyes were excised after instillation at 1 hour, and the conjunctiva and cornea were collected. Distribution of these emulsions in extraocular tissues was evaluated by a confocal laser scanning microscopy. CE and NE containing indomethacin as a model drug was also instilled into rabbit eyes, and the tear fluid was then collected. The drug concentration in the tear was measured by HPLC. The elimination profile of the drug in tear was analyzed using SCPF model.

Results and Discussion: : Fluorescent distribution for these emulsions was clearly observed by a confocal laser scanning microscopy. Fluorescence of CE in conjunctiva was more intensive than that of NE. We, therefore, visually confirmed distribution of CE in conjunctiva. In the tear fluid, the drug level of CE was higher than that of NE. These elimination profiles were well described by the SCPF model, and the model parameters, parallel flow ratio α, side flow rate ß and side–capacity volume ration γ, were also evaluated. ß and γ were almost the same for these emulsions. However, α of CE was smaller than that of NE. These values of parameters suggest that CE might be subject to stay longer in conjunctiva than NE.

Keywords: conjunctiva • cornea: tears/tear film/dry eye • microscopy: confocal/tunneling 
© 2006, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept