May 2006
Volume 47, Issue 13
ARVO Annual Meeting Abstract  |   May 2006
Evaluation of Ocular Tolerability of Repetitive Corneal–Scleral Iontophoresis in Pigmented Rabbits
Author Affiliations & Notes
  • P.–P. Elena
    Iris Pharma, La Gaude, France
  • P. Roy
    EyeGate Pharma, Paris, France
  • K. Viaud
    Iris Pharma, La Gaude, France
  • T. Caillaud
    Iris Pharma, La Gaude, France
  • Footnotes
    Commercial Relationships  P. Elena, None; P. Roy, EyeGate Pharma, E; K. Viaud, None; T. Caillaud, None.
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 5094. doi:
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      P.–P. Elena, P. Roy, K. Viaud, T. Caillaud; Evaluation of Ocular Tolerability of Repetitive Corneal–Scleral Iontophoresis in Pigmented Rabbits . Invest. Ophthalmol. Vis. Sci. 2006;47(13):5094.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : To investigate the potential of iontophoresis for repetitive delivery of drugs, pigmented rabbits received a serial trans–corneal–scleral iontophoretic applications of Eyegate device during 14 days. Animals were observed along the application period and after a recovery period of 14 days and the tolerance of the treatment was evaluated.

Methods: : Pigmented rabbits (n=10) were administered with one 5 min at 12 O’clock trans–corneal–scleral iontophoretic administration with Eyegate device filled with BSS. No intensity (0 mA) was applied in left eyes and 2.5 mA applied in right eyes. This administration was performed one time per day during 14 days. Ocular evaluations using Draize scale, McDonald–Shadduck scale, lissamine green test, confocal corneal microscope and ERG were performed. At day 15, 5 animals were sacrificed and eyes were sampled for ocular histology examination. The other 5 animals were observed during a recovery period of 14 days and were sacrificed with same sampling on day 29.

Results: : Following administration, we find some redness of conjunctiva, some conjunctiva chemosis and corneal haze with low scores on both eyes and the main score was 1 (scale: 0–4). The other eye structures were not modified or affected. All the effects observed were reversible. Fluorescein staining was observed on both eyes for 8 animals, mainly at day 15 and were not reversible on both eyes for 3 animals out of 5 during the recovery period. No other ocular modification was observed with this test. The lissamine green test showed transitory suffering from cornea 5 min after administration on both eyes. No or little suffering from cornea was observed each day before iontophoretic administration. No effect was observed with confocal corneal microscope except some scratches of the epithelium. Histopathological examinations on the first set of 5 animals only showed an epithelial abrasion of bulbar conjunctiva in both eyes. The animals sacrificed on day 29, showed an epithelial abrasion of bulbar conjunctiva only on left eye. A total recovery of conjunctival epithelium in right eye was observed.

Conclusions: : The multiple trans–corneal–scleral iontophoretic administrations during 14 days induces some redness, chemosis of the conjunctiva and corneal haze in both eyes and minimal epithelial abrasion of the bulbar conjunctiva for some animals, independent from the current applied and reversible.

Keywords: ocular irritancy/toxicity testing • drug toxicity/drug effects 

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