May 2006
Volume 47, Issue 13
ARVO Annual Meeting Abstract  |   May 2006
The Role of Intravitreal t–PA, in an Experimental Model of Retinal Vein Thrombosis
Author Affiliations & Notes
  • E.V. Christodoulakis
    Ophthalmology, University of Crete, Medical School, Heraklion, Greece
  • S. Antimisiaris
    Pharmacology, University of Patras, Patras, Greece
  • E. Grigoryan
    Developmental Biology, Koltzov Institute, Russian Academy of Science, Moscow, Russian Federation
  • S. Harisis
    Ophthalmology, University of Crete, Medical School, Heraklion, Greece
  • M.K. Tsilimbaris
    Ophthalmology, University of Crete, Heraklion, Greece
  • Footnotes
    Commercial Relationships  E.V. Christodoulakis, None; S. Antimisiaris, None; E. Grigoryan, None; S. Harisis, None; M.K. Tsilimbaris, None.
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 5168. doi:
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      E.V. Christodoulakis, S. Antimisiaris, E. Grigoryan, S. Harisis, M.K. Tsilimbaris; The Role of Intravitreal t–PA, in an Experimental Model of Retinal Vein Thrombosis . Invest. Ophthalmol. Vis. Sci. 2006;47(13):5168.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : To evaluate the use of intravitreal t–PA within and without liposomes, in an experimental rabbit model of retinal vessel photothrombosis.

Methods: : 5 rabbits, 10 eyes were been thrombosed using a photodynamic technique, and the natural course of thrombosis were monitored for 45 days. Two similar groups were prepared, and intravitreal injections of 60 µg tPA and 15 µg of tPA encapsulated in liposomes were performed immediately after thrombosis, and the course of thrombosis was compared to controls. T–PA penetration to the vascular lumen was studied with immunohistochemistry assay.

Results: : Retinal vessels remain occluded for more than 45 days after photothrombosis. Although differences were noticed at injection groups as micro–haemorrhages and PVD, no evidence of circulation restore was present. Immunohistochemistry shown no definite evidence of t–PA, nor t–PA entrapped in liposomes, penetration into the vascular lumen.

Conclusions: : t–PA and t–PA encapsulated in liposomes, seems not to be able to change the natural course of retinal vein thrombosis, in this experimental model.

Keywords: vascular occlusion/vascular occlusive disease • pharmacology • photodynamic therapy 

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