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N. Tsurumaru, K. Teruya, J. Sueda, M. Arai, R. Yamakawa; Application of Seprafilm® for Trabeculectomy in Rabbit Eyes . Invest. Ophthalmol. Vis. Sci. 2006;47(13):5487.
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© ARVO (1962-2015); The Authors (2016-present)
To determine the efficacy of Seprafilm® (Genzyme Corporation, Cambridge, MA), a bioresorbable translucent membrane composed of two chemically modified anionic polysaccharides, sodium hyaluronate and carboxymethylcellulose, to prevent postoperative adhesion during trabeculectomy in rabbits.
All surgeries were carried out with the animals under general anesthesia. Five male albino rabbits comprised the study group undergoing trabeculectomy with Seprafilm® . After a fornix–based conjunctival incision was made, a 3×3 mm scleral flap was created. Trabeculectomy was performed at the scleral spur followed by iridectomy. A 3×2 mm Seprafilm® sheet was placed beneath the scleral flap. Another 5×6 mm Seprafilm® sheet was put over the scleral flap. The scleral flap and cojunctival wound were sutured with 10–0 nylon. In the 5 control rabbits, the same procedures were performed without Seprafilm®. Mitomycin C was not applied in any case. Intraocular pressure (IOP) was measured with a Tonopen® in both eyes and examinations of the anterior segment were performedon days 1 and 3 and each week postoperatively.
In the study group, the mean IOP of the operated eyes was 9.9±0.6 mmHg and that of the fellow eyes was 13.2±0.8 mmHg 4 weeks postoperatively(p<0.001). In the control group, the mean IOP of the operated was 11.9±0.8 mmHg and that of the fellow eyes was 12.2±0.9 mmHg 1 week postoperatively (p=ns). A vascular bleb formed in the study group for 4 weeks postoperatively but disappeared in the control group on day 5 postoperatively.
Seprafilm® can assist filtering bleb formation after trabeculectomy prevent sclera–sclera and sclera–conjunctival adhesions postoperatively.
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