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M. Ayaki, K. Shimada, H. Nishihara, S. Yaguchi, A. Iwasawa; Corneal Toxicity of the Disinfectant – Potential Use for Surgical Instruments . Invest. Ophthalmol. Vis. Sci. 2006;47(13):5558.
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© ARVO (1962-2015); The Authors (2016-present)
Ortho–phthalaldehyde (CidexROPA, Johnson and Johnson KK) is a medical disinfectant commonly used for endoscope. Recommended protocol is five minutes’ soaking and three times washing for standard disinfection. We investigated corneal toxicity of CidexROPA to explore its potential use for ophthalmic surgical instruments since it is time–, space–, and cost–saving..
We made primary culture of porcine corneal endothelium and human corneal endothelium retrieved from eye bank eyes. The other cells, human corneal epithelium (SIRC cells), bovine corneal epithelium (BCE C/D–1b cells), and rabbit corneal epithelium (RC1 cells) were commercially available. We harvested 104 cells/100µl in 96 culture well and observed cell survival with WST–1 assay for endothelium and MTT assay for epithelium after two days’ incubation in media with diluted disinfectant. Cell survival was calculated as a ratio to the value of control (distilled water). Each assay was replicated 16 times.
The human corneal endothelial survival rates were 80% in 100x dilution, and over 90% in 500x or more dilutions. Porcine endothelial survival was 100% at 100x or more dilutions. All of epithelial survival was 100% at 100x–500x or more dilutions.
Corneal toxicity of CidexROPA seemed to be within safety range after dilution by routine washing. It could be used for preparation of selected surgical instruments under urgent or under–equipped circumstances.
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