May 2006
Volume 47, Issue 13
ARVO Annual Meeting Abstract  |   May 2006
Inflammatory Cytokine Gene Expression of Lacrimal Gland of Botulinum Toxin–B Induced Mouse Dry Eye Model
Author Affiliations & Notes
  • C. Park
    Ophthalmology, Wilmer, Baltimore, MD
  • K. Lekhanont
    Ophthalmology, Wilmer, Baltimore, MD
  • W.–S. Lee
    Ophthalmology, Wilmer, Baltimore, MD
  • R.S. Chuck
    Ophthalmology, Wilmer, Baltimore, MD
  • Footnotes
    Commercial Relationships  C. Park, None; K. Lekhanont, None; W. Lee, None; R.S. Chuck, None.
  • Footnotes
    Support  NIH Grant EY000412–04
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 5597. doi:
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      C. Park, K. Lekhanont, W.–S. Lee, R.S. Chuck; Inflammatory Cytokine Gene Expression of Lacrimal Gland of Botulinum Toxin–B Induced Mouse Dry Eye Model . Invest. Ophthalmol. Vis. Sci. 2006;47(13):5597.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : To investigate the inflammatory cytokine gene expression in lacrimal gland of botulinum toxin–B induced mouse dry eye model.

Methods: : Twenty one female, 8–week–old CBA/J mice were used. Fifteen mice were injected with botulinum toxin–B(BTX–B, MyoblocTM, Elan Pharmaceuticals Inc., South San Francisco, CA, 0.05ml, 20mU) into both lacrimal glands, three mice were was injected with saline(0.05ml) into both lacrimal glands, and another three mice were used as control without any injection. Corneal fluorescein staining was evaluated at 1, 2, 4 and 8 weeks after injection. Lacrimal glands were harvested at 2, 4 and 8 week after injection and gene microarray analysis for inflammatory cytokine and receptors (Oligo GEArrayTM, catalog no. OM 011, Bioscience Corp., Frederick, MD) was performed.

Results: : Slight increased corneal fluorescein staining was observed in BTX–B injected mice at weeks 1, 2 and 4 after injection compared to non–injected and saline injected mice. Macrophage migration inhibitory factor(MIF), Toll interacting protein(Tollip), complement component 3(C3), interleukin 10 receptor beta(IL10rb) and interleukin 12A(IL12a) were stongly expressed and interleukin 1 bete(Il1b), interleukin 1 receptor 1(Il1r1), interleukin 6 receptor alpha(IL6ra), interleukin 20(Il20), interkeukin 6 signal transducer(Il6st), chemokine receptor 2(Ccr2), Fc receptor for IgG(Fcgr1), Toll like receptor 3(Tlr3) and chemokine ligand 14(Cxcl14) were weakly expressed. The expression of these genes showed no significant difference among non–, saline and BTX–B injected mice at 2week.

Conclusions: : We report gene expression change of inflammatory cytokines and their receptors after BTX–B injection into mouse lacrimal gland. This characteristic change supports the ocular–lacrimal gland interaction in keratoconjuctivitis sicca.

Keywords: gene microarray • drug toxicity/drug effects • inflammation 

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