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B.d. Furlani, F.M. Penha, M. Maia, E. Dib, O. Magalhães, Jr., M.E. Farah, V.C. Lima, H. Palacios, E.H. Fraymüller, R.L. Smith; Effects of Subretinal Injection of Indocyanine Green and Infracyanine in Rabbit Eyes . Invest. Ophthalmol. Vis. Sci. 2006;47(13):5681.
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To evaluate the effects os subretinal injection of indocyanine green (ICG), infracyanine (IF), and balanced salt solution (BSS) in rabbits.
Ten animals were submitted to vitrectomy and subretinal injection of 0.02 ml of: 1) 0.05% ICG (270 mOsm); 2) 0.5% IF (285 mOsm) and 3) BSS (300mOsm), which was tested as a control. Animals were followed by 6, 12, 24 hours and 14 days following the surgical procedure by fluorescein angiography and indirect binocular ophthalmoscopy; histological evaluation was performed by light and electron microscopy.
All the subretinal blebs were flat 24 hours following the surgical procedure. The subretinal injection of 0.05% ICG caused photoreceptor inner segments (PIS) and outer segments(POS) damage as well as outer nuclear layer (ONL) and also retinal pigment epithelium (RPE) damage; the longer the follow–up, the more severe was the injury. The subretinal injection of 0.5% IF (285mOsm) resulted in PIS and ONL histological abnormalities 24 hours and 14 days after surgery. The fluorescein angiography showed window defects in positions that ICG and IF were injected into the subretinal space, which were more prominent in the ICG subretinal area. Subretinal injection of BSS (300mOsm) resulted in minimal abnormalities of the POS during all follow–up.
The subretinal injection of 0.05% ICG resuts in retinal damage which was more important than subretinal injection of 0.5% IF . These findings may explain the RPE abnormalities observed in some cases following internal limiting membrane peeling for macular hole surgery and emphasizes that care must be taken to avoid subretinal migration of these substances through the macular hole during vitreoretinal surgery. The use of infracyanine in vitreoretinal surgery deserves further investigation.
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