May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Time Course of Retinal Progenitor Cell Differentiation Following Subretinal Transplantation Into Retinal Degenerate Rats
M. Wang; G. Qiu; M.J. Seiler; S. Deb; T. Vien; Z. Chen; S.R. Sadda
Author Affiliations & Notes
  • M. Wang
    Ophthalmology, University of Southern California, Los Angeles, CA
    Ophthalmology,
    Doheny Eye Institute, Los Angeles, CA
  • G. Qiu
    Ophthalmology, University of Southern California, Los Angeles, CA
    Retina–Ophthalmology,
    Doheny Eye Institute, Los Angeles, CA
  • M.J. Seiler
    Ophthalmology, University of Southern California, Los Angeles, CA
    Cell and Neurobiology,
    Doheny Eye Institute, Los Angeles, CA
  • S. Deb
    Ophthalmology, University of Southern California, Los Angeles, CA
    Retina–Ophthalmology,
    Doheny Eye Institute, Los Angeles, CA
  • T. Vien
    Ophthalmology, University of Southern California, Los Angeles, CA
    Retina–Ophthalmology,
    Doheny Eye Institute, Los Angeles, CA
  • Z. Chen
    Ophthalmology, University of Southern California, Los Angeles, CA
    Retina–Ophthalmology,
    Doheny Eye Institute, Los Angeles, CA
  • S.R. Sadda
    Ophthalmology, University of Southern California, Los Angeles, CA
    Retina–Ophthalmology,
    Doheny Eye Institute, Los Angeles, CA
  • Footnotes
    Commercial Relationships  M. Wang, None; G. Qiu, None; M.J. Seiler, None; S. Deb, None; T. Vien, None; Z. Chen, None; S.R. Sadda, None.
  • Footnotes
    Support  NIH EY03040, Foundation Fighting Blindness, Foundation for Retinal Research, Fletcher Jones Foundation
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 5748. doi:
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      M. Wang, G. Qiu, M.J. Seiler, S. Deb, T. Vien, Z. Chen, S.R. Sadda; Time Course of Retinal Progenitor Cell Differentiation Following Subretinal Transplantation Into Retinal Degenerate Rats . Invest. Ophthalmol. Vis. Sci. 2006;47(13):5748.

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Abstract

Purpose: : To study the differentiation profile of retinal progenitor cells (RPCs) following subretinal transplantation into retinal degenerate(RD) rats at different time points, and to further determine whether the extent of degeneration of the host retina modulates cell fate determination.

Materials and Methods: : RPCs were isolated from embryonic day 17 human alkaline phosphatase (hPAP)–positive rat retina, and expanded in serum–free defined culture medium supplemented with EGF and bFGF. RPCs at passage 2 were transplanted into the subretinal space of one eye of RD rats at different stages of degeneration (ages P28 and P48). In control animals, PBS was injected in the same fashion in one eye. Rats were sacrificed at post–transplantation week 1, 2, 3 and 4. Immunohistochemistry for various retinal markers including rhodopsin, PKC, calretinin, calbindin, and GFAP was performed.

Results: : Transplanted RPCs organized into a multicellular layer in the subretinal space as early as post–operative week one. Extensive migration of grafted cells into the remaining host retina was observed in some transplanted eyes at various follow–up time points. Both rhodopsin and GFAP immunoreactive cells were seen within the grafts in post–operative week one, demonstrating that in this in vivo microenvironment, RPCs retain the potential for differentiation toward multiple cell fates. Of interest, rhodopsin expression appeared to increase over time. By postoperative week 4, the majority of the grafted cells were rhodopsin immunoreactive. No significant increase in GFAP immunoreactive cells in the graft was observed over this 4–week time. Expression of PKC, calbindin, calretinin and synapsin within the graft varied over time. A similar differentiation profile of RPC grafts was seen in both P28 and P48 day old recipients.

Conclusions: : The differentiation and maturation of RPCs following subretinal transplantation in RD rats appears to show a time course of events. This could provide opportunities for influencing cell fate determination. The differentiation profile of E17 derived RPCs was not affected by the stage of the host retinal degeneration, suggesting an important role for intrinsic cues.

Keywords: retina • transplantation • differentiation 
© 2006, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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