May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Morphological and Functional Rescue in a Rodent Model of Retinal Degeneration With Umbilical Tissue Derived Cells
S.K. Mistry; D. Messina; I. Harris; T. Kihm; Y. Sauve; S. Wang; S. Girman; T. Holmes; L. Bin; R. Lund
Author Affiliations & Notes
  • S.K. Mistry
    Johnson & Johnson Internal Ventures, Centocor, Radnor, PA
  • D. Messina
    Johnson & Johnson Internal Ventures, Centocor, Radnor, PA
  • I. Harris
    Johnson & Johnson Internal Ventures, Centocor, Radnor, PA
  • T. Kihm
    Center for Biomaterials and Advanced Technologies, Ethicon Inc, Sommerville, NJ
  • Y. Sauve
    University of Utah, Moran Eye Center, Salt Lake City, UT
  • S. Wang
    University of Utah, Moran Eye Center, Salt Lake City, UT
  • S. Girman
    University of Utah, Moran Eye Center, Salt Lake City, UT
  • T. Holmes
    University of Utah, Moran Eye Center, Salt Lake City, UT
  • L. Bin
    University of Utah, Moran Eye Center, Salt Lake City, UT
  • R. Lund
    University of Utah, Moran Eye Center, Salt Lake City, UT
  • Footnotes
    Commercial Relationships  S.K. Mistry, Johnson & Johnson, E; Inventor, P; D. Messina, Johnson & Johnson, E; Inventor, P; I. Harris, Johnson & Johnson, E; Inventor, P; T. Kihm, Ethicon Inc., E; Inventor, P; Y. Sauve, None; S. Wang, None; S. Girman, None; T. Holmes, None; L. Bin, None; R. Lund, None.
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 5788. doi:
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      S.K. Mistry, D. Messina, I. Harris, T. Kihm, Y. Sauve, S. Wang, S. Girman, T. Holmes, L. Bin, R. Lund; Morphological and Functional Rescue in a Rodent Model of Retinal Degeneration With Umbilical Tissue Derived Cells . Invest. Ophthalmol. Vis. Sci. 2006;47(13):5788.

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Abstract

Purpose: : To examine efficacy of a defined cell population of human umbilical tissue derived cells (hUDC) to rescue photoreceptors from degeneration.

Methods: : hUDC were isolated and maintained in culture. They were characterized in detail. A suspension of cells was injected into the subretinal space of postnatal day (P) 21 pigmented Royal College of Surgeons (RCS) rats. Animals were tested for a range of visual responses at P60, P90 & P120 after which eyes were fixed for histology.

Results: : : hUDC were essentially homogeneous and bound surface antigens characteristic of stromal cells such as CD10, 13 and 90, but not markers of hematopoietic or endothelial cells such as CD34 or CD31. hUDC from this process achieved >40 population doublings without karyotypic changes and were determined to secrete neurotrophic, neuroprotective and angiogenic factors such as BDNF, IL6, IL8 and bFGF via ELISA. After subretinal injection, they significantly preserved ERG responses at P60 and P90 compared to rats receiving cells derived from placental tissue, dermal fibroblasts, sham injection and untreated eyes. Further testing at P100 –120 showed that they sustained a mean performance significantly higher than sham–injected and unoperated controls on optomotor test for acuity and that visual perimetry recording from the superior colliculus examining luminance threshold responses also gave significantly improved performance over controls. Histological examination of rats receiving hUDC injections showed evidence of donor cell survival and substantial photoreceptor rescue over controls.

Conclusions: : hUDC preserve photoreceptors and a range of visual functions in the RCS rat model; they are also readily obtainable in large numbers. As such they provide an efficient potential cell therapy treatment for patients with Retinitis Pigmentosa.

Keywords: retinal degenerations: cell biology • photoreceptors: visual performance • retina 
© 2006, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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