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T. Morimoto, J.–S. Choi, T. Miyoshi, Y. Fukuda, Y. Tano, T. Fujikado; Effects of Transcorneal Electrical Stimulation on the Survival of Photoreceptors in Royal College Surgeons Rats . Invest. Ophthalmol. Vis. Sci. 2005;46(13):183.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: Previously, we demonstrated that transcorneal electrical stimulation (TES) promotes the survival of axotomized retinal ganglion cells in adult rats. In this study, we investigated the effect of TES on the survival of photoreceptors in Royal College Surgeons (RCS) rats. Methods: Three–week–old RCS rats received TES by using contact lens electrode once a week for 4 weeks. TES was applied for 1 hour. The stimuli of TES were biphasic pulses (1 ms/phase, 40 Hz frequency) at the current intensity of 100 µA. At 7 weeks of age, RCS rats were examined for photoreceptor survival using light microscope. Visual function was assessed by electroretinography (ERG). Results: Morphologic analysis of the retinas of 7 week–old RCS rats showed that, the mean thickness of outer nuclear layer (ONL) in the eyes treated with TES was significantly thicker than that in the eyes with sham stimulation (15.5 ± 2.16 vs 10.1 ± 1.54 µm (mean ± SD), P < 0.05). Electrophysiological studies showed that in 7–week–old RCS rats treated with TES, the threshold of scotopic threshold response (STR) was 0.5 log cd/m2 lower than that in the eyes with sham stimulation (P < 0.05). Conclusions: TES prolongs the survival of photoreceptors and delays the loss of retinal function against the retinal degeneration in RCS rats. These findings indicate that TES would be a potential therapeutic method for the diseases of photoreceptor cells such as retinitis pigmentosa.
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