May 2005
Volume 46, Issue 13
ARVO Annual Meeting Abstract  |   May 2005
Proteomic Analysis of Diabetic and Non–Diabetic Rat Retinae
Author Affiliations & Notes
  • G.J. Quin
    Clinical Ophthalmology, University Sydney, Sydney, Australia
  • M.C. Gillies
    Clinical Ophthalmology, University of Sydney, Sydney, Australia
  • Footnotes
    Commercial Relationships  G.J. Quin, None; M.C. Gillies, None.
  • Footnotes
    Support  ORIA Research Grant
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 406. doi:
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      G.J. Quin, M.C. Gillies; Proteomic Analysis of Diabetic and Non–Diabetic Rat Retinae . Invest. Ophthalmol. Vis. Sci. 2005;46(13):406.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose:The aim of this project was to identify protein changes induced by short term diabetes in the rat retina. Methods: Neuro–sensory retinae were pooled from Dark Agouti male rats after 10 weeks of streptozotocin–induced diabetes. Two–dimensional gel electrophoresis technology provided separation of solubilized proteins. After UV staining with Sypro–ruby (Bio–Rad)and Commassie Blue, PDQuest (Bio–Rad) software enabled comparison of protein spot profiles. Protein spots of interest were cut and peptide spectra generated using MALDI TOF–TOF (ABI 4700) and Q–TOF Ultima (Applied Biosystems) instruments. Spectra were compared to those of known proteins in the NCBI database for identification. Results: Two–dimension gel profiles revealed a significant number of upregulated and down–regulated proteins. Diabetes was found to induce changes in retinal Crystallin subtype expression, Heat shock proteins, Retinaldehyde binding proteins, mitochondrial metabolic enzymes, chaperone proteins, adolase, tyrosine, and glycolytic metabolic enzymes . Conclusions: Two–dimensional gel electrophoresis coupled with mass spectrometry technology has allowed identification of multiple changes at the protein level in an animal model of diabetic retinopathy.

Keywords: diabetic retinopathy • proteomics • proteins encoded by disease genes 

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