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G.K. Berry, J.B. Reed; The Effect of Sedimentation on the Concentration of Triamcinolone Acetonide . Invest. Ophthalmol. Vis. Sci. 2005;46(13):492.
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Purpose: To evaluate the effect of sedimentation on the concentration of triamcinolone acetonide (Kenalog–40) at various time intervals. Methods: Forty–two 10mL vials of Kenalog–40 (Bristol–Myers Squibb, each containing 40mg/mL of triamcinolone acetonide) were divided into six groups containing seven vials. Using a 22G needle on a tuberculin syringe, a 0.5mL sample was withdrawn from each vial. In the control group, 0.4mL was immediately wasted from each syringe and the remaining 0.1mL then evaluated by high performance liquid chromatography to determine the mass of triamcinolone acetonide without sedimentation. In each of the other five groups, 0.5mL was similarly withdrawn from each vial. Individual syringes were then oriented vertically to allow sedimentation for 2.5 minutes, 5 minutes, 10 minutes, 20 minutes or 40 minutes. A supernatant volume of 0.4mL was then wasted from each syringe and the remaining 0.1mL was analyzed by high performance liquid chromatography to determine the mass of triamcinolone acetonide at each sedimentation time. Results: In the control group, the mean mass of triamcinolone acetonide in 0.1mL was 3.93mg (95%CI 3.30–4.56). With precipitation the mean mass of triamcinolone acetonide was 2.97mg at 2.5 minutes (95%CI 2.35–3.60), 5.10mg at 5 minutes (95%CI 4.47–5.72), 7.91mg at 10 minutes (95%CI 7.29–8.54), 9.57mg at 20 minutes (95%CI 8.94–10.20), and 11.30mg at 40 minutes (95%CI 10.66–11.91). Conclusions: By allowing Kenalog–40 to precipitate for ten minutes or more, a significant and predictable increase in concentration may be obtained.
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