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A.P. Wells, R. Wallis, J.G. Crowston, G. Reeves; Indocyanine Green With Mitomycin C: Effect on Cytotoxicity in Human Tenon's Fibroblasts . Invest. Ophthalmol. Vis. Sci. 2005;46(13):59.
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Purpose: The addition of indocyanine green (ICG) to mitomycin–C (MMC) may permit localization of antimetabolite during trabeculectomy. This would facilitate estimation of treatment area, recognition of accidental application to inappropriate tissues, and sponge localization. This study examined the effect of ICG on MMC–mediated inhibition of Tenon's fibroblast proliferation. Methods: Fibroblast monolayers were exposed to either MMC (0.4mg/ml in PBS) or PBS containing indocyanine green (0.065%, 0.125%, 0.250% and 0.5% in 200µl PBS) for 5 minutes. Controls were exposed for 5 minutes to MMC, PBS or culture medium containing no ICG. Following treatment, the monolayers were washed and incubated in culture medium for 24, 48, 72hr and 1 week periods. At the conclusion of each incubation period, cell number was assessed using the CyQUANT® cell proliferation assay. Results: The presence of ICG alone, at concentrations ranging from 0.0625% to 0.500%, had no effect on the rate of fibroblast proliferation. There was no significant difference in cell number between controls and monolayers exposed to all concentrations of ICG (range 32 to 34 x 10^2 (p <0.1)) up to one week post exposure. In contrast, MMC treatment resulted in a significant reduction in viable fibroblast number (8.4 ± 0.13 x 10^2) and loss of cell viability was observed. At all concentrations tested, ICG in combination with MMC did not alter fibroblast number up to 1 week compared to MMC alone (8.5 ± 0.05 and 8.4 ± 0.12 x 10^2 respectively). Conclusions: ICG had no effect on fibroblast proliferation. ICG did not potentiate or diminish the effect of mitomycin C on Tenon's capsule fibroblast proliferation.
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