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H. Liu, O. Mohamed, D. Dufort, V.A. Wallace; The Role of Wnt Signaling in Retinal Development . Invest. Ophthalmol. Vis. Sci. 2005;46(13):596.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: Wnts are secreted glycoproteins that play critical roles in patterning and morphogenesis of many tissues and organs during embryonic development. Several Wnts and Wnt pathway components are expressed in the developing eye, however, the functional significance of Wnt signaling during retinal development is largely unknown. The purpose of this project is to assess retinal development in response to modulating beta–catenin dependent Wnt signaling. Methods: Ectopic expression of Wnt2b and a constitutively active isoform of beta–catenin is induced by electroporation of embryonic retinal explants from wildtype and Tcf/Lef1–LacZ Wnt reporter transgenic mice. Expression of the transgenes, as well as the effect of ectopic Wnt signaling on retinal development, is monitored by in situ hybridization and immunohistochemistry. Results: Ectopic expression of Wnt2b affects the presentation of neural retinal progenitor specific antigens. We show that ectopic Wnt signaling activates Wnt reporter gene expression in retinal explants from Tcf/Lef1–LacZ mice and alters retinal progenitor gene expression. Conclusions: Wnt signaling pathway involves in the developmental processes in mammalian retina.
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