May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
The Role of Wnt Signaling in Retinal Development
H. Liu; O. Mohamed; D. Dufort; V.A. Wallace
Author Affiliations & Notes
  • H. Liu
    Department of Biochemistry Microbiology and Immunology, University of Ottawa Eye Institute, Molecular Medicine Program, Ottawa Health Research Institute, Ottawa, ON, Canada
  • O. Mohamed
    Department of Obstetrics and Gynecology, Royal Victoria Hospital, Mcgill University Health Center, Montreal, PQ, Canada
  • D. Dufort
    Department of Obstetrics and Gynecology, Royal Victoria Hospital, Mcgill University Health Center, Montreal, PQ, Canada
  • V.A. Wallace
    Department of Biochemistry Microbiology and Immunology, University of Ottawa Eye Institute, Molecular Medicine Program, Ottawa Health Research Institute, Ottawa, ON, Canada
  • Footnotes
    Commercial Relationships  H. Liu, None; O. Mohamed, None; D. Dufort, None; V.A. Wallace, None.
  • Footnotes
    Support  Foundation Fighting Blindness Canada
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 596. doi:
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      H. Liu, O. Mohamed, D. Dufort, V.A. Wallace; The Role of Wnt Signaling in Retinal Development . Invest. Ophthalmol. Vis. Sci. 2005;46(13):596.

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Abstract

Abstract: : Purpose: Wnts are secreted glycoproteins that play critical roles in patterning and morphogenesis of many tissues and organs during embryonic development. Several Wnts and Wnt pathway components are expressed in the developing eye, however, the functional significance of Wnt signaling during retinal development is largely unknown. The purpose of this project is to assess retinal development in response to modulating beta–catenin dependent Wnt signaling. Methods: Ectopic expression of Wnt2b and a constitutively active isoform of beta–catenin is induced by electroporation of embryonic retinal explants from wildtype and Tcf/Lef1–LacZ Wnt reporter transgenic mice. Expression of the transgenes, as well as the effect of ectopic Wnt signaling on retinal development, is monitored by in situ hybridization and immunohistochemistry. Results: Ectopic expression of Wnt2b affects the presentation of neural retinal progenitor specific antigens. We show that ectopic Wnt signaling activates Wnt reporter gene expression in retinal explants from Tcf/Lef1–LacZ mice and alters retinal progenitor gene expression. Conclusions: Wnt signaling pathway involves in the developmental processes in mammalian retina.

Keywords: retinal development • cell-cell communication • gene/expression 
© 2005, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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