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T.H. Ko, V.J. Srinivasan, M.T. Carvalho, M. Wojtkowski, S.–E. Bursell, J. Lem, J.S. Duker, J.S. Schuman, J.G. Fujimoto; Three Dimensional Retinal Imaging of Small Animals With High–speed, Ultrahigh Resolution Optical Coherence Tomography . Invest. Ophthalmol. Vis. Sci. 2005;46(13):1051.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To introduce high–speed, ultrahigh resolution optical coherence tomography for performing non–invasive three dimensional imaging and mapping of the retina in small animal models. Methods: A high–speed, ultrahigh resolution OCT system using new "spectral/Fourier domain" detection has been developed for small animal imaging. This technology can achieve imaging speeds of over 12,500 axial scans per second, an improvement of ∼100x over previous ultrahigh resolution OCT systems. The system is interfaced with a microscope delivery system for focusing and scanning the OCT beam on the animal retina. Using a broadband femtosecond laser light source, an axial image resolution of ∼2 µm is achieved. High–speed, ultrahigh resolution retinal imaging is performed in mouse and rat models. Retinal imaging is performed with multiple raster scans in order to enable three dimensional imaging and comprehensive coverage of the retina. Results: High–speed, ultrahigh resolution OCT enables high resolution and high pixel density imaging of the retina and the visualization of all major intraretinal layers. Improved coverage of the animal retina is also achieved with a raster scan imaging protocol that can acquire 128 images, each containing 512 axial scans, in ∼5 seconds. An OCT fundus image can be generated from the three–dimensional OCT data, which enables precise registration of individual OCT images to retinal fundus features. Conclusions: High–speed, ultrahigh resolution OCT enables imaging of retinal architectural morphology in small animal models. OCT fundus images allow precise registration of OCT images to retinal features. The ability to perform three dimensional imaging with comprehensive coverage of the retina enables detailed visualization and quantitation of retinal structure allowing noninvasive, repeated imaging to track disease progression, monitoring retinal pathologic changes in response to novel therapeutic interventions, and reducing the need for sacrifice and histology. This capability can acclerate the translation from basic research studies in rats and mice into the clinical care arena by characterizing unique surrogate clinical end points.
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