May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Three Dimensional Retinal Imaging of Small Animals With High–speed, Ultrahigh Resolution Optical Coherence Tomography
T.H. Ko; V.J. Srinivasan; M.T. Carvalho; M. Wojtkowski; S.–E. Bursell; J. Lem; J.S. Duker; J.S. Schuman; J.G. Fujimoto
Author Affiliations & Notes
  • T.H. Ko
    Electrical Engineering and Computer Science and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA
  • V.J. Srinivasan
    Electrical Engineering and Computer Science and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA
  • M.T. Carvalho
    Electrical Engineering and Computer Science and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA
  • M. Wojtkowski
    Electrical Engineering and Computer Science and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA
    New England Eye Center, Tufts–New England Medical Center, Boston, MA
  • S.–E. Bursell
    Joslin Diabetes Center, Harvard Medical School, Boston, MA
  • J. Lem
    New England Eye Center, Tufts–New England Medical Center, Boston, MA
  • J.S. Duker
    New England Eye Center, Tufts–New England Medical Center, Boston, MA
  • J.S. Schuman
    UPMC Eye Center, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA
  • J.G. Fujimoto
    Electrical Engineering and Computer Science and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA
  • Footnotes
    Commercial Relationships  T.H. Ko, None; V.J. Srinivasan, None; M.T. Carvalho, None; M. Wojtkowski, None; S. Bursell, None; J. Lem, None; J.S. Duker, None; J.S. Schuman, Carl Zeiss Meditec P; J.G. Fujimoto, Carl Zeiss Meditec P.
  • Footnotes
    Support  NIH EY11289–19, EY13178–05, P30–EY13078, NSF ECS–0119452, AFOSR FA9550–040–1–0046,Carl Zeiss Meditec
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 1051. doi:
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      T.H. Ko, V.J. Srinivasan, M.T. Carvalho, M. Wojtkowski, S.–E. Bursell, J. Lem, J.S. Duker, J.S. Schuman, J.G. Fujimoto; Three Dimensional Retinal Imaging of Small Animals With High–speed, Ultrahigh Resolution Optical Coherence Tomography . Invest. Ophthalmol. Vis. Sci. 2005;46(13):1051.

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Abstract

Abstract: : Purpose: To introduce high–speed, ultrahigh resolution optical coherence tomography for performing non–invasive three dimensional imaging and mapping of the retina in small animal models. Methods: A high–speed, ultrahigh resolution OCT system using new "spectral/Fourier domain" detection has been developed for small animal imaging. This technology can achieve imaging speeds of over 12,500 axial scans per second, an improvement of ∼100x over previous ultrahigh resolution OCT systems. The system is interfaced with a microscope delivery system for focusing and scanning the OCT beam on the animal retina. Using a broadband femtosecond laser light source, an axial image resolution of ∼2 µm is achieved. High–speed, ultrahigh resolution retinal imaging is performed in mouse and rat models. Retinal imaging is performed with multiple raster scans in order to enable three dimensional imaging and comprehensive coverage of the retina. Results: High–speed, ultrahigh resolution OCT enables high resolution and high pixel density imaging of the retina and the visualization of all major intraretinal layers. Improved coverage of the animal retina is also achieved with a raster scan imaging protocol that can acquire 128 images, each containing 512 axial scans, in ∼5 seconds. An OCT fundus image can be generated from the three–dimensional OCT data, which enables precise registration of individual OCT images to retinal fundus features. Conclusions: High–speed, ultrahigh resolution OCT enables imaging of retinal architectural morphology in small animal models. OCT fundus images allow precise registration of OCT images to retinal features. The ability to perform three dimensional imaging with comprehensive coverage of the retina enables detailed visualization and quantitation of retinal structure allowing noninvasive, repeated imaging to track disease progression, monitoring retinal pathologic changes in response to novel therapeutic interventions, and reducing the need for sacrifice and histology. This capability can acclerate the translation from basic research studies in rats and mice into the clinical care arena by characterizing unique surrogate clinical end points.

Keywords: imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • imaging/image analysis: non-clinical • retina 
© 2005, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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