May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Multiphoton Imaging of Porcine Eyes
H.–Y. Tan; S.–W. Teng; J.–L. Peng; H.–H. Lin; H.–Y. Wu; W. Lo; Y. Sun; W.–C. Lin; S.–J. Lin; C.–Y. Dong
Author Affiliations & Notes
  • H.–Y. Tan
    Institute of Medical Engineering, Pathology,
    National Taiwan University, Taipei, Taiwan Republic of China
    Ophthalmology, Chang Gung Memorial Hospital, TaoYuan, Taiwan Republic of China
  • S.–W. Teng
    Physics, Dermatology,
    National Taiwan University, Taipei, Taiwan Republic of China
  • J.–L. Peng
    Life Science,
    National Taiwan University, Taipei, Taiwan Republic of China
  • H.–H. Lin
    Physics, Dermatology,
    National Taiwan University, Taipei, Taiwan Republic of China
  • H.–Y. Wu
    Physics, Dermatology,
    National Taiwan University, Taipei, Taiwan Republic of China
  • W. Lo
    Physics, Dermatology,
    National Taiwan University, Taipei, Taiwan Republic of China
  • Y. Sun
    Physics, Dermatology,
    National Taiwan University, Taipei, Taiwan Republic of China
  • W.–C. Lin
    Institute of Medical Engineering, Pathology,
    National Taiwan University Hospital, Taipei, Taiwan Republic of China
  • S.–J. Lin
    Physics, Dermatology,
    National Taiwan University Hospital, Taipei, Taiwan Republic of China
  • C.–Y. Dong
    Physics, Dermatology,
    National Taiwan University, Taipei, Taiwan Republic of China
  • Footnotes
    Commercial Relationships  H. Tan, None; S. Teng, None; J. Peng, None; H. Lin, None; H. Wu, None; W. Lo, None; Y. Sun, None; W. Lin, None; S. Lin, None; C. Dong, None.
  • Footnotes
    Support  NSC 93–3112–B–002–033 (national science council, Taiwan)
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 1081. doi:
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      H.–Y. Tan, S.–W. Teng, J.–L. Peng, H.–H. Lin, H.–Y. Wu, W. Lo, Y. Sun, W.–C. Lin, S.–J. Lin, C.–Y. Dong; Multiphoton Imaging of Porcine Eyes . Invest. Ophthalmol. Vis. Sci. 2005;46(13):1081.

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Abstract

Abstract: : Purpose: To demonstrate the feasibility of using multiphoton microscopy in imaging ocular surface. Methods: The porcine eyes used for imaging were immersed in PBS buffer for viewing. The home–built multiphoton microscope used for this study was constructed from a commercial upright microscope (Nikon E800). Using a 40x, NA 0.8 water immersion objective (Fluor), the 780 nm photons from a titanium sapphire laser were used to induced multiphoton autofluorescence and second–harmonic generation(SHG) signals from different positions and depths of porcine ocular surface. Results:Without extrinsic fluorescent molecules, we were able to image the ocular surface. The cornea and limbal epithelial cells (autofluorescence), as well as the well–organized corneal stromal collagen fibers (SHG) are visible . The imaging results were compatible with the histological morphology. Conclusions: We demonstrate an excellent imaging of porcine ocular surface using multiphoton induced fluorescence and SHG signals, without the introduction of extrinsic fluorescent molecules. With additional developements, multiphoton microscopy may in future be applicated for in vivo investigation of ophthalmologic pathologies.

Keywords: microscopy: light/fluorescence/immunohistochemistry • cornea: basic science • imaging/image analysis: non-clinical 
© 2005, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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