Purchase this article with an account.
G. Takeuchi, D. Borja, F. Manns, N. Ziebarth, D. Denham, J.–M. Parel; Measurement of the Optical Quality of Post–Mortem Lenses During Simulated Accommodation . Invest. Ophthalmol. Vis. Sci. 2005;46(13):736.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: To measure the modulation transfer functions of post–mortem human and monkey crystalline lenses during simulated accommodation. Methods: A benchtop optical system was constructed to measure the single–pass point–spread function (PSF) of post–mortem lens specimens mounted in a manual lens stretching system (Parel et al, ARVO 2004). The lens specimens include the zonules, ciliary body and sclera. The optical system consists of a fiber–coupled super–luminescent diode that produces a 3mm collimated beam from a single mode fiber. The collimated beam is transmitted through the lens specimen. The intensity distribution at the focus of the lens corresponds to the lens PSF. A system of two plano–convex anti–reflection coated achromatic doublet lenses creates a magnified image of the crystalline lens PSF onto a high–resolution monochrome CCD camera. A computer program processes the data and calculates the MTF from the PSF. The imaging lens is mounted on an axial positioning stage for focus adjustment and the specimen is mounted on a lateral positioning stage for centering. After calibration using a microscope objective of known characteristics, pilot experiments were conducted on 2 human crystalline lenses, one in the unstretched state only and one in the unstretched state and stretched state (2mm stretching). Changes in optical quality were quantified by comparing the cutoff frequency of the MTF. Results: The cutoff frequency for an 80 year–old eye unstretched and stretched increased from 90 to106 lines/mm respectively while the 50% frequency remained constant at 14 lines/mm. The cutoff and the 50% frequencies for a 39 year–old human lens in the unstretched state were 15 and 50 lines/mm respectively. Conclusions: This pilot study demonstrates the feasibility of measuring the optical quality of crystalline lenses during stretching to simulate accommodation. Pilot results indicate that there are changes in the optical quality of post–mortem lenses during simulated accommodation. Support: NIH F31EY015395–01; NIH 5R01EY014225; Vision Cooperative Research Centre, Sydney Australia; Florida Lions Eye Bank; NIH center grant P30–EY014801; Research to Prevent Blindness; Henri and Flore Lesieur Foundation
This PDF is available to Subscribers Only