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D.B. Denham, D. Borja, G. Regev, A. Rosen, N. Ziebarth, R. Augusteyn, A. Ho, F. Manns, B. Holden, J.–M. Parel; Human and Monkey ex vivo Lens Biometry Using Shadowphotogrammetry . Invest. Ophthalmol. Vis. Sci. 2005;46(13):739.
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© ARVO (1962-2015); The Authors (2016-present)
To compute the biometric properties of excised intact fresh human, cynomologus (cynos) and rhesus lenses.
31 human (40–99 y/o), 39 rhesus (0.75–16 y/o) and 48 cynos (2.2–10.4 y/o) lenses immersed in an isotonic fluid–filled cell were digitally recorded on a shadowphotogrammetric system at 20X (∼13µm linear resolution) designed to measure lens equatorial diameter (D) and sagittal thickness (T) (Rosen AM et al, ARVO 2002). Assuming rotational symmetry, crystalline lenses can be described as 2 separate semi–oblate spheroids of revolution (for anterior and posterior surfaces with semi–minor axes of bA and bP respectively). Using this model, exact formulas were used to compute lens volume (V), capsule surface area (S), polar perimeter (P), maximal diameter of the "equivalent flattened" capsule (Ø), polar cross–sectional area (CSA) and shape parameters (SP). Linear regression and Mean±SD were calculated.
The ratio bA/bP remained constant with age. The average values and slopes at mean age (MA) were:
Shadowphotogrammetry permits accurate biometry of fresh crystalline lenses. Although there are differences in the size of the lenses between species and with age, there are no significant differences in the shape parameters (SP1 & SP2).
: NIH R01EY14225 & F31EY015395, Florida Lions Eye Bank; Australian Government’s CRC Scheme; NIH center grant P30–EY014801; Research to Prevent Blindness; Henri and Flore Lesieur Foundation; Norma Kenyon PhD, Pat Gullett DVM, Daniel Rothen DVM.
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