May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Human and Monkey ex vivo Lens Biometry Using Shadowphotogrammetry
Author Affiliations & Notes
  • D.B. Denham
    Ophthalmic Biophysics Center, Bascom Palmer Eye Inst, Univ. of Miami School of Medicine, Miami, FL
    Dept. of Biomedical Engineering, Univ. of Miami College of Engineering, Coral Gables, FL
  • D. Borja
    Ophthalmic Biophysics Center, Bascom Palmer Eye Inst, Univ. of Miami School of Medicine, Miami, FL
    Dept. of Biomedical Engineering, Univ. of Miami College of Engineering, Coral Gables, FL
  • G. Regev
    Ophthalmic Biophysics Center, Bascom Palmer Eye Inst, Univ. of Miami School of Medicine, Miami, FL
  • A. Rosen
    Ophthalmic Biophysics Center, Bascom Palmer Eye Inst, Univ. of Miami School of Medicine, Miami, FL
    Dept. of Biomedical Engineering, Univ. of Miami College of Engineering, Coral Gables, FL
  • N. Ziebarth
    Ophthalmic Biophysics Center, Bascom Palmer Eye Inst, Univ. of Miami School of Medicine, Miami, FL
    Dept. of Biomedical Engineering, Univ. of Miami College of Engineering, Coral Gables, FL
  • R. Augusteyn
    Vision Cooperative Research Center, Sydney, Australia
  • A. Ho
    Vision Cooperative Research Center, Sydney, Australia
  • F. Manns
    Ophthalmic Biophysics Center, Bascom Palmer Eye Inst, Univ. of Miami School of Medicine, Miami, FL
    Dept. of Biomedical Engineering, Univ. of Miami College of Engineering, Coral Gables, FL
  • B. Holden
    Vision Cooperative Research Center, Sydney, Australia
    School of Optometry & Vision Science, Univ. of New South Wales, Sydney, Australia
  • J.–M. Parel
    Ophthalmic Biophysics Center, Bascom Palmer Eye Inst, Univ. of Miami School of Medicine, Miami, FL
    Dept. of Biomedical Engineering, Univ. of Miami College of Engineering, Coral Gables, FL
  • Footnotes
    Commercial Relationships  D.B. Denham, None; D. Borja, None; G. Regev, None; A. Rosen, None; N. Ziebarth, None; R. Augusteyn, None; A. Ho, None; F. Manns, None; B. Holden, None; J. Parel, None.
  • Footnotes
    Support  NIH R01EY14225 & F31EY015395; Australia Gov't CRC Scheme; NIH center grant P30–EY014801; RPB; FLEB
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 739. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      D.B. Denham, D. Borja, G. Regev, A. Rosen, N. Ziebarth, R. Augusteyn, A. Ho, F. Manns, B. Holden, J.–M. Parel; Human and Monkey ex vivo Lens Biometry Using Shadowphotogrammetry . Invest. Ophthalmol. Vis. Sci. 2005;46(13):739.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract
 
Abstract:
 

To compute the biometric properties of excised intact fresh human, cynomologus (cynos) and rhesus lenses.

 

31 human (40–99 y/o), 39 rhesus (0.75–16 y/o) and 48 cynos (2.2–10.4 y/o) lenses immersed in an isotonic fluid–filled cell were digitally recorded on a shadowphotogrammetric system at 20X (∼13µm linear resolution) designed to measure lens equatorial diameter (D) and sagittal thickness (T) (Rosen AM et al, ARVO 2002). Assuming rotational symmetry, crystalline lenses can be described as 2 separate semi–oblate spheroids of revolution (for anterior and posterior surfaces with semi–minor axes of bA and bP respectively). Using this model, exact formulas were used to compute lens volume (V), capsule surface area (S), polar perimeter (P), maximal diameter of the "equivalent flattened" capsule (Ø), polar cross–sectional area (CSA) and shape parameters (SP). Linear regression and Mean±SD were calculated.

 

The ratio bA/bP remained constant with age. The average values and slopes at mean age (MA) were:

 

 

Volume and surface area showed an increase with age for all species. These data are in good agreement with previous studies (Glasser, Tehrani, Pierscionek, Richburg, Galand, Scammon) which used different measurement techniques and assumptions to model the intact lens and the "equivalent flattened" capsular bag.

 

Shadowphotogrammetry permits accurate biometry of fresh crystalline lenses. Although there are differences in the size of the lenses between species and with age, there are no significant differences in the shape parameters (SP1 & SP2).

 

: NIH R01EY14225 & F31EY015395, Florida Lions Eye Bank; Australian Government’s CRC Scheme; NIH center grant P30–EY014801; Research to Prevent Blindness; Henri and Flore Lesieur Foundation; Norma Kenyon PhD, Pat Gullett DVM, Daniel Rothen DVM.

 

 
Keywords: imaging/image analysis: non-clinical • aging • cataract 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×