Purchase this article with an account.
L.M. Meyer, X. Dong, V. Mody, M. Kakar, S. Löfgren, A. Wegener, P. Söderberg; Dose Response Function and MTD for UVR–B–Induced Cataract Development in the C57BL/6 Mouse . Invest. Ophthalmol. Vis. Sci. 2005;46(13):834.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: To investigate the dose response function for forward light scattering in the C57BL/6 mouse lens after in vivo exposure to UVR–B 300 nm and to establish the maximum tolerable dose (MTD) for avoidance of UVR–B induced cataract in the mouse lens. Methods: A total of 80 six weeks old female mice were divided into four dose groups (n = 20). The animals received, in vivo depending on the dose group unilaterally 0, 2, 4, or 8 kJ/m2 UVR–B for 15 minutes. Animals in the 0 dose group received no radiation and functioned as a control. The radiation output of the UVR– source had MAX at 302.6 nm with 4.5 nm [FWHM]. The animals were sacrificed two days after exposure. Light scattering in the exposed and the contra lateral non–exposed lenses was measured quantitatively. Morphological lens changes were documented using grid and dark field illumination photography. Results: Two days after exposure, cataract occurred in the exposed lenses in all dose groups except the control group. With increased UVR–dose the opacities became more intense. Mainly anterior subcapsular but also cortical and nuclear opacities were identified in the mice that received 2, 4, and 8 kJ/m2 UVR–B. Exposed lenses scattered light more intensely than non exposed lenses. The light scattering difference between exposed and control lenses was significant at 4 and 8 kJ/m2 UVR–B. The intensity of forward light scattering increased with rising UVR–dose between 2 and 8 kJ/m2. MTD for the mouse lens was estimated at 3.2 kJ/m2 UVR–B. Conclusions: The intensity of forward light scattering in the C57BL/6mouse lens increases exponentially with increasing UVR–B dose between 2 and 8 kJ/m2.
This PDF is available to Subscribers Only