May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Assessments of Cleaning Efficacy for Contact Lens Multi–Purpose Disinfecting Solutions
Author Affiliations & Notes
  • B.–S. Hong
    Consumer Products Research, Alcon Laboratories, Inc., Fort Worth, TX
  • P. Stauffer
    Consumer Products Research, Alcon Laboratories, Inc., Fort Worth, TX
  • D.L. Meadows
    Consumer Products Research, Alcon Laboratories, Inc., Fort Worth, TX
  • Footnotes
    Commercial Relationships  B. Hong, Alcon Laboratories, Inc. E; P. Stauffer, Alcon Laboratories, Inc. E; D.L. Meadows, Alcon Laboratories, Inc. E.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 914. doi:
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    • Get Citation

      B.–S. Hong, P. Stauffer, D.L. Meadows; Assessments of Cleaning Efficacy for Contact Lens Multi–Purpose Disinfecting Solutions . Invest. Ophthalmol. Vis. Sci. 2005;46(13):914.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To assess the passive cleaning efficacy of various multipurpose solutions (MPS) for hydrogel contact lenses using an in vitro lysozyme deposition model. Methods: The solutions assessed included AQuify®, ReNu® MoistureLocTM, ReNu MultiPlus®, Complete®, Complete® Moisture Plus, SolocareTM, SolocareTM Plus, Opti–Free®, Opti–Free® Express® and a test formulation. Acuvue 2 lenses (FDA Group IV) were used to deposit with an in vitro lysozyme deposit model. Each lens was soaked with a 5 ml lysozyme solution (1.5mg/ml in phosphate buffered saline, pH 7.4) at 37oC for 24 hours. No rubbing/rinsing was applied to the cleaning regimen. The lysozyme remained in the lens after the passive soaking with MPS was extracted with an extraction solvent composing of acetonitrile/trifluoroacetic acid/water in a volume ratio of 500/1/500. Quantitation of the extracted lysozyme was conducted by a fluorescence spectrophotometer with an excitation/emission setting at 280nm/346nm. Results: Time course studies demonstrated that the cleaning efficacy increased with the duration of soaking; 6 hr and overnight (16 hr) soaking achieved more significant cleaning than 2 and 4 hr soaking. Differences in cleaning efficacies were observed among various MPS; they ranged from approximately 6% to 44 %. The overall cleaning efficacy for all MPS were increased after 7–day cleaning cycle. In both cases, Opti–Free® Express® exceeded all other MPS in cleaning efficacy, whereas AQuify® and ReNu MoistureLocTM provided the lowest cleaning. These in vitro results agree with ex vivo clinical studies that have tested the cleaning efficacies of the various products. Conclusions: Using Acuvue hydrogel lenses deposited with an in vitro lysozyme deposition model, we are able to differentiate the cleaning efficacy of various MPS. Opti–Free® Express® was demonstrated to exceed all other MPS in cleaning of the contact lenses.

Keywords: contact lens 
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