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J.M. Hooymans, P.B. J. Vermeltfoort, T.G. Van Kooten, G.M. Bruinsma, H.C. Van der Mei, H.J. Busscher; Bacterial Transmission From Contact Lenses to ex vivo Porcine Eyes . Invest. Ophthalmol. Vis. Sci. 2005;46(13):929.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: This study aimed to quantify the transmission of the hydrophilic Staphylococcus aureus 835 and hydrophobic Pseudomonas aeruginosa #3 from three types of contact lenses, differing in surface hydrophobicity and roughness, to ex vivo porcine eyes. Methods: Contact lenses were inoculated in a bacterial suspension for 30 min and then placed on an ex vivo porcine eye. After 16 h of contact between lens and eye, confocal laser scanning microscopy was used to determine the number of bacteria on the lens and eye surface for the calculation of transmission percentages. Results: Transmission percentages were significantly different for both bacterial strains from an etafilcon A lens and balafilcon A lens (p=0.006 and 0.04, respectively). Percentages varied from 51 to 68% for the hydrophobic P. aeruginosa and from 54 to 82% for the hydrophilic S. aureus strain, depending on the contact lens type involved. Both strains were transferred the least from the most hydrophilic and roughest lens, made of lotrafilcon A, although this was only statistically significant for S. aureus. Conclusions: Bacterial transmission to a porcine eye was proven to differ for various types of contact lenses and was the least from a hydrophilic and rough lens type.
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