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P.C. Lu, L.S. Wu, P. Wu; Comprehensive Allelotyping of Human Pterygium . Invest. Ophthalmol. Vis. Sci. 2005;46(13):950.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:To search for specific alterations in human pterygiae and understand the molecular events leading to the pathogenesis of human pterygiae by examination of loss of heterogenesity (LOH) in human pterygiae.Methods:Forty eight pairs of samples were collected from 48 patients from the department of ophthalmology , Chiayi Chang Gung Memorial Hospital, Taiwan. For those who have bilateral pterygiae, only one eye will be used in the study. The pterygium specimens were obtained by excision. We matched pterygium tissue and corresponding peripheral blood cell (PBC) DNA samples by using the ABI PRISM Linkage Mapping Sets MD–10 (400 makers) and PCR. Data of genotypes are scored for LOH using Gene Mapper (ABI) software.Results:The highest percentage of LOH was found for a locus in 4q35 (STR marker D4S426, 17%, 5 in 30 informative cases) This region was not reported in previous studies and nearby the FAT and caspase 3 tumor suppression genes. The other aberrant locus is 13q14 (STR marker D13S153, 7%, 3 in 45 informative cases) which region close to RB gene. This result should help in identifying new genes whose loss of function contributes to the development of pterygium. Conclusions:LOH in pterygium are less than that in other tumor. Chromosome abnormality may not most important cause for pterygium. DNA repair genes polymorphism or mutation should be considered as a major genetic factor.
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