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C.A. Cox, A. Martinez, T.W. Reid, N. Dushku, C. Jaworski, M. John–Aryankalayil, D.A. Carper; Doxycycline's Effect on the Angiogenic Potential of Pterygial Epithelial Cells . Invest. Ophthalmol. Vis. Sci. 2005;46(13):959.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To determine the angiogenic potential of human pterygial epithelial cells and doxycycline's effect on neovascularization induced by these cells using the directed in vivo angiogenesis assay (DIVAA). Methods: The angiogenic potential of fourth passage human pterygial epithelial cells was compared with basic fibroblast growth factor (bFGF) in Matrigel for a positive control and PBS in Matrigel for a negative control using DIVAA (approved NCI animal protocol). In this assay, silicone capsules (angioreactors) containing Matrigel with PBS, bFGF or 10,000 human pterygial epithelial cells were implanted under the skin of anesthetized nude mice. After 11 days, fluorescein isothiocyanate–dextran (FITC–d) was injected into the tail veins of the mice, and ten minutes later they were euthanized and the extent of neovascularization was quantified using a spectrofluorimeter (excitation 485 nm, emission 510 nm.). The mean relative fluorescence for six replicates was determined; Student's t–test was used to analyze the data. In a separate DIVAA experiment, doxycycline at three different concentrations was added to the angioreactors in combination with pterygial cells to determine the effect on angiogenesis. Six replicates were compared for each group as above.Results:Human pterygial epithelial cells were significantly angiogenic when compared to Matrigel with PBS (p<0.01) and Matrigel with bFGF (p<0.05). Doxycycline significantly inhibited angiogenesis in the mid–concentration (500 micrograms/ml) p=0.003, but not in the lowest or highest concentrations.Conclusions: Human pterygial epithelial cells were very angiogenic using an in vivo model, and a mid–range dose of doxycycline inhibited neovascularization in this model. This may eventually be beneficial to patients' vision by reducing the growth and/or recurrence of not only pterygia, but other angiogenic ocular diseases.
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