May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Distribution of Amyloid Precursor Protein and ß–Amyloid in Ocular Hypertensive C57BL/6 Mouse Eyes
Author Affiliations & Notes
  • D. Goldblum
    Department of Ophthalmology, University Bern/ Inselspital, Bern, Switzerland
  • A. Kauer
    Department of Ophthalmology, University Bern/ Inselspital, Bern, Switzerland
  • S.J. McKinnon
    Ophthalmology and Cellular & Structural Biology, UTHSCSA, San Antonio, TX
  • L. Kasmala
    Ophthalmology and Cellular & Structural Biology, UTHSCSA, San Antonio, TX
  • B.E. Frueh
    Department of Ophthalmology, University Bern/ Inselspital, Bern, Switzerland
  • Footnotes
    Commercial Relationships  D. Goldblum, None; A. Kauer, None; S.J. McKinnon, None; L. Kasmala, None; B.E. Frueh, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 1266. doi:
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    • Get Citation

      D. Goldblum, A. Kauer, S.J. McKinnon, L. Kasmala, B.E. Frueh; Distribution of Amyloid Precursor Protein and ß–Amyloid in Ocular Hypertensive C57BL/6 Mouse Eyes . Invest. Ophthalmol. Vis. Sci. 2005;46(13):1266.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Abstract:
 

To determine the distribution of APP and ß–amyloid (Aß) in the retinas of induced ocular hypertensive eyes of C57BL/6 DBA/2J.

 

Ocular hypertension was induced in one eye of 10 three months old C57BL/6 mice. 6 weeks later retinas were fixed with 4% paraformaldehyde and processed for immunohistochemistry. Antibodies used included a polyclonal antibody to the C–terminus of Aß 40 (Novartis 17–40/23) and a polyclonal antibody to the APP ectodomain (Novartis 474). Immunohistochemical stained tissue was graded (0 to 4) using light microscopy. Distribution and semiquantitative expression of APP and Aß in retinas was determined in a blinded fashion, and correlated to the IOP.

 

The mean staining intensity of 10 eyes in each group was as follows.

 

 

Aß correlated significantly with IOP whereas elevated (statistically not significant) APP levels in ocular hypertensive mice could be found. Disruption of the homeostatic properties of secreted APP with consecutive Aß cytotoxicity might be a contributing factor of producing apoptotic cell death in mouse RGCs.

 

 
Keywords: immunohistochemistry • ganglion cells • apoptosis/cell death 
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