Purchase this article with an account.
D. Goldblum, A. Kauer, S.J. McKinnon, L. Kasmala, B.E. Frueh; Distribution of Amyloid Precursor Protein and ß–Amyloid in Ocular Hypertensive C57BL/6 Mouse Eyes . Invest. Ophthalmol. Vis. Sci. 2005;46(13):1266.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To determine the distribution of APP and ß–amyloid (Aß) in the retinas of induced ocular hypertensive eyes of C57BL/6 DBA/2J.
Ocular hypertension was induced in one eye of 10 three months old C57BL/6 mice. 6 weeks later retinas were fixed with 4% paraformaldehyde and processed for immunohistochemistry. Antibodies used included a polyclonal antibody to the C–terminus of Aß 40 (Novartis 17–40/23) and a polyclonal antibody to the APP ectodomain (Novartis 474). Immunohistochemical stained tissue was graded (0 to 4) using light microscopy. Distribution and semiquantitative expression of APP and Aß in retinas was determined in a blinded fashion, and correlated to the IOP.
The mean staining intensity of 10 eyes in each group was as follows.
This PDF is available to Subscribers Only