May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Modulation of Glutamate Toxicity in Retinal Ganglion Cells by Extracellular Matrix
Author Affiliations & Notes
  • K. Chatzipanteli
    Ophthalmology, University of Miami School of Medicine, Bascom Palmer Eye Institute, FL
  • M.E. Fini
    Ophthalmology, University of Miami School of Medicine, Bascom Palmer Eye Institute, FL
  • Footnotes
    Commercial Relationships  K. Chatzipanteli, None; M.E. Fini, None.
  • Footnotes
    Support  Walter G Ross Foundation
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 1321. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      K. Chatzipanteli, M.E. Fini; Modulation of Glutamate Toxicity in Retinal Ganglion Cells by Extracellular Matrix . Invest. Ophthalmol. Vis. Sci. 2005;46(13):1321.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose:Previous studies have reported that kainic acid–induced apoptosis of retinal ganglion cells correlates with specific degradation of laminin from the underlying inner limiting membrane by an increased gelatinolytic metalloproteinase activity. This study evaluated the effects of matrix and matrix components on retinal ganglion cell line (RGC–5) growth and viability. Since NMDA excitotoxicity mediates the death of retinal ganglion cells (RGCs) in glaucoma we studied the effects of different inner limiting membrane components on RGC–5 viability in the presence of glutamate. Methods:RGC–5 cells were cultured in media containing 10% fetal calf serum. Viable attached cells were assessed using the neutral red dye uptake assay. Proliferation was quantified by 5’–bromo–2’–deoxyuridine (BrDU) incorporation into RGC–5 cells, immunostaining at 17 hours after plating on laminin and matrigel matrices. Components evaluated included type IV collagen, fibronectin, laminin, and matrigel infiltrated with growth factors. We tested the effect of glutamate on RGC–5 cells grown in the above media in the presence and absence of 5 mM glutamic acid, incubated for 24 hours. Results: Analysis of the BrDU incorporation into RGC–5 cells suggested that matrigel increased growth by accelerating cell proliferation and viability in comparison to laminin. The efficacy of neuroprotection by the different matrix components and matrix itself in the presence of glutamate were as follows: fibronectin 95.87%>Collagen 90.42%>uncoated 89.5%>laminin 77.96%>matrigel 72.34%. Conclusions: These results suggest that matrigel is superior to laminin in promoting RGC–5 attachment and survival. However, it also potentiates glutamate toxicity.

Keywords: extracellular matrix • excitatory neurotransmitters • retinal culture 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×