May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Immunohistochemical Evaluation of the Extracellular Matrix in the Human Trabecular Meshwork of Steroid Induced Glaucoma Specimens
Author Affiliations & Notes
  • A. Tawara
    Department of Ophthalmology, Univ of Occup & Environ Health, Kitakyushu–shi, Japan
  • S. Tanaka
    Department of Ophthalmology, Univ of Occup & Environ Health, Kitakyushu–shi, Japan
  • N. Tou
    Department of Ophthalmology, Univ of Occup & Environ Health, Kitakyushu–shi, Japan
  • T. Kubota
    Department of Ophthalmology, Univ of Occup & Environ Health, Kitakyushu–shi, Japan
  • Y. Harada
    Department of Ophthalmology, Univ of Occup & Environ Health, Kitakyushu–shi, Japan
  • N. Hirose
    Sakka Eye Clinic, Kitakyushu–shi, Japan
  • Footnotes
    Commercial Relationships  A. Tawara, None; S. Tanaka, None; N. Tou, None; T. Kubota, None; Y. Harada, None; N. Hirose, None.
  • Footnotes
    Support  Grant–in–aid from the Ministry of Education, Science & Culture of the Japanese Government 1659177
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 1353. doi:
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      A. Tawara, S. Tanaka, N. Tou, T. Kubota, Y. Harada, N. Hirose; Immunohistochemical Evaluation of the Extracellular Matrix in the Human Trabecular Meshwork of Steroid Induced Glaucoma Specimens . Invest. Ophthalmol. Vis. Sci. 2005;46(13):1353.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To immunohistochemically examine the localization of the extracellular matrix in the trabecular meshwork (TM) of steroid induced glaucoma (SG) specimens. Methods:We both morphologically and immunohistochemically examined 2 trabeculectmized specimens from 2 cases of SG and 1 from a case of primary open angle glaucoma (POAG), and 1 normal TM from an enucleated eye due to conjunctival malignant melanoma. For the morphological study, sections cut on an ultra–microtome were observed with an electron microscope. For the light microscopic immunohistochemistry analyses, frozen or paraffin embedded sections were stained by the avidin–biotin complex method using either anti–MYOC, anti–heparan sulfate type proteoglycan (HSPG), anti–fibronectin, anti–type IV collagen or anti–type VI collagen antibody. Double immunostaining using anti–MYOC and one of the other antibodies listed above was done using the immunofluorescence method. Results: According to the electron microscopic findings, accumulations of basement membrane–like materials were observed in the outer TM of SG. In SG specimens, both HSPG and type IV collagen antibodies showed a greater degree of staining in the outer TM than either the POAG or normal specimens, while the other antibodies including MYOC did not. Using double immunohistochemistry, MYOC was found to be partly colocalized with HSPG and type IV collagen in the TM of SG. Conclusions: The localization of the extracellular matrixes is different in the TM of SG than in that of normal individuals or POAG patients.

Keywords: trabecular meshwork • extracellular matrix • immunohistochemistry 
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