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A. Tawara, S. Tanaka, N. Tou, T. Kubota, Y. Harada, N. Hirose; Immunohistochemical Evaluation of the Extracellular Matrix in the Human Trabecular Meshwork of Steroid Induced Glaucoma Specimens . Invest. Ophthalmol. Vis. Sci. 2005;46(13):1353.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To immunohistochemically examine the localization of the extracellular matrix in the trabecular meshwork (TM) of steroid induced glaucoma (SG) specimens. Methods:We both morphologically and immunohistochemically examined 2 trabeculectmized specimens from 2 cases of SG and 1 from a case of primary open angle glaucoma (POAG), and 1 normal TM from an enucleated eye due to conjunctival malignant melanoma. For the morphological study, sections cut on an ultra–microtome were observed with an electron microscope. For the light microscopic immunohistochemistry analyses, frozen or paraffin embedded sections were stained by the avidin–biotin complex method using either anti–MYOC, anti–heparan sulfate type proteoglycan (HSPG), anti–fibronectin, anti–type IV collagen or anti–type VI collagen antibody. Double immunostaining using anti–MYOC and one of the other antibodies listed above was done using the immunofluorescence method. Results: According to the electron microscopic findings, accumulations of basement membrane–like materials were observed in the outer TM of SG. In SG specimens, both HSPG and type IV collagen antibodies showed a greater degree of staining in the outer TM than either the POAG or normal specimens, while the other antibodies including MYOC did not. Using double immunohistochemistry, MYOC was found to be partly colocalized with HSPG and type IV collagen in the TM of SG. Conclusions: The localization of the extracellular matrixes is different in the TM of SG than in that of normal individuals or POAG patients.
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