May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Intravitreal Triamcinolone Acetonide Augments the Rod Electroretinogram (ERG) b–Wave in Rabbit
Author Affiliations & Notes
  • B. Lei
    Mason Eye Institute,
    Veterinary Medicine and Surgery,
    University of Missouri–Columbia, Columbia, MO
  • D. Dierks
    Mason Eye Institute,
    University of Missouri–Columbia, Columbia, MO
  • K. Zhang
    Veterinary Medicine and Surgery,
    University of Missouri–Columbia, Columbia, MO
  • D.P. Hainsworth
    Mason Eye Institute,
    University of Missouri–Columbia, Columbia, MO
  • Footnotes
    Commercial Relationships  B. Lei, None; D. Dierks, None; K. Zhang, None; D.P. Hainsworth, None.
  • Footnotes
    Support  Research to Prevent Blindness, Inc. University of Missouri Research Board
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 1461. doi:
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      B. Lei, D. Dierks, K. Zhang, D.P. Hainsworth; Intravitreal Triamcinolone Acetonide Augments the Rod Electroretinogram (ERG) b–Wave in Rabbit . Invest. Ophthalmol. Vis. Sci. 2005;46(13):1461.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Promising results have been observed with the use of intravitreal triamcinolone to improve the visual acuity of patients with diabetic macular edema and age–related macular degeneration. We investigate the clinical, histological, and electroretinographic effects of intravitreal triamcinolone acetonide suspension and its commercial supernatant in the rabbit retina. Methods: Eighteen New Zealand white rabbits were separated into 3 groups; each receiving an intravitreal injection in the right eye. Group 1 received 4 mg of commercially available triamcinolone acetonide; Group 2 received an equal volume (0.1 ml) of the steroid supernatant; and Group 3 received 4 mg of triamcinolone acetonide with the supernatant removed and replaced with balanced salt solution. The left eyes of the rabbits served as the control groups. The concentration of triamcinolone in the supernatant was measured by high–performance liquid chromatography (HPLC). Anterior segment, dilated funduscopic, dark– and light–adapted electroretinographic (ERG) examinations were performed at baseline and at 3–4 and 6–7 days after injection. Enucleated eyes were examined histologically. Results: Ocular examination revealed no differences among the groups. Eyes that received intravitreal triamcinolone (Groups 1 and 3) had a 10∼25% increase in dark–adapted ERG a– and b–wave amplitudes, as compared to control eyes, when subjected to stimulation with moderate– to high–flash intensities. A comparison of ERG responses of injected and control eyes revealed that the average ratio of injected eyes showed a significantly higher dark–adapted b–wave amplitude in Group 1 when compared to Group 2 at –0.83 log cd*s/m2 (p<0.05). No histological differences were observed between study and control eyes. Conclusions: Intravitreal injection of 4 mg of triamcinolone acetonide is not toxic to the rabbit retina. Triamcinolone may augment the rod–driven ERG responses, suggesting a mechanism by which visual function may improve in diseased retina.

Keywords: drug toxicity/drug effects • corticosteroids • electroretinography: non-clinical 
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